生物醫學研究所

課程資訊

學年/學期	112/2
授課教師	李國銘
開課單位	生醫所博士班
年級-班別	1-甲
科目代號(學分)	EMM114(2)
課程名稱	分子病毒方法學概論
開課序號	61413
主要開課序號
修課學生	本校: 3人；外校: 0人
人數限制	2~60
上課時間地點	星期 開始 / 結束 教室 [代碼說明] 星期一 3 (10:10) ~ 4 (12:00) 每週上課
核心能力	按此查詢
備註	英語授課，M0860-2

教學大綱

教科書	Lecture slides
教科書ISBN	0000000000
參考書	Molecular cloning : a laboratory manual by Michael Green et al., 4th Edition (2012), Fields Virology: Emerging Viruses by Peter Howley et al., 7th Edition (2020), Molecular Biology by Robert Weaver, 5th Edition (2011), Molecular Virology of Human Pathogenic Viruses by Wang-Shick Ryu (2017)
課程講義網址
整體教學目標	Aim to let the attendees have a comprehensive understanding of both the traditional and advanced techniques applied in the studies of the molecular virology
教學方法	Lectures, discussion, and student presentation
課程簡介	以lecture的形式介紹實驗室進行病毒學研究常用的技術以及方法。除了傳統的分生以及生化技術外，也會導入新穎的次世代實驗方法。課程會以病毒的分子檢測為出發點，當我們遭遇未知的病毒感染源，如何快速辨別可能的病原體？除了傳統偵測病毒核酸或是蛋白質表現的分子實驗方法，以及以細胞培養為基礎進行病毒定量以及抗病毒藥物效性的相關實驗外，課程還會討論近年發展出的研究技術如蛋白質、代謝體學，次世代/第三代定序，以及基因篩選/編輯等新穎的技術及其如何應用於病毒以及病毒宿主間交互作用的相關研究成果
彈性學習	The flexible courses are scheduled to be hold on 2024/06/10 (Mon), and 2024/06/17 (Mon), 10:10-12:00. 1. Physical or online course to be announced 2. Discussion for two Videos: 1. Approaches for using protein-protein interaction networks for biological discovery (European Bioinformatics Institute - EMBL-EBI); 2. Single Cell Sequencing (iBiology Techniques)
成績考核	Final presentation: 60% Classroom performance and discussion: 30% Attendance: 10%
師生互動時間	Monday 12:00 (pm) to 13:00 Kuo-Ming Lee, g39002007@mail.cgu.edu.tw, x-3727

教學進度

項次	教學主題
1	1. Physical course 2. Topic: Introduction: Methodology in Molecular Virology and Diagnosis 3. Lecturer: Kuo-Ming Lee
2	1. Physical course 2. Topic: Molecular Cloning: DNA Technology and Evolution of the Central Dogma 3. Lecturer: Kuo-Ming Lee
3	1. Physical course 2. Topic: Basic Protein Analysis: Antibody-based Techniques in Molecular, Biochemical, and Cellular Biology 3. Lecturer: Kuo-Ming Lee
4	1. Physical course 2. Topic: Old-school Nucleic Acid Analysis: Northern and Southern Blotting, Nuclease-based Analyses, and In Vitro Assays 3. Lecturer: Kuo-Ming Lee
5	1. Physical course 2. Topic: Basic Nucleic Acid Analysis: PCR, RT-PCR and PCR-based Nucleic Acid Amplification Tests (NAAT) 3. Lecturer: Kuo-Ming Lee
6	1. Physical course 2. Topic: Application of CRISPR and Isothermal Amplification in NAATs of Molecular Diagnosis 3. Lecturer: Kuo-Ming Lee
7	1. Physical course 2. Topic: Next and Third-generation Sequencing in the post Genomic Era 3. Lecturer: Kuo-Ming Lee
8	1. Physical course 2. Topic: Bioinformatic and Evolutionary Analysis: Tools in Epidemiology of SARS-CoV-2 3. Lecturer: Yu-Nong Gong
9	1. Physical course 2. Topic: Cell Culture and Various Nucleic Acid Delivery Systems 3. Lecturer: Kuo-Ming Lee
10	1. Physical course 2. Topic: Derivatives from RNA Viruses as Prospective Tools for Clinical and Basic Research 3. Lecturer: Kuo-Ming Lee
11	1. Physical course 2. Topic: mRNA Vaccine and other Nucleic Acid Therapeutics 3. Lecturer: Kuo-Ming Lee
12	1. Physical course 2. Topic: Virus Propagation, Viral Titer Measurement and Antiviral Assay 3. Lecturer: Yu-An Kung
13	1. Physical course 2. Topic: Serological Tests in Molecular Virology 3. Lecturer: Yu-An Kung
14	1. Physical course 2. Topic: CRISPR/Cas System in the Genetic Screening 3. Lecturer: Kuo-Ming Lee
15	1. Physical course 2. Topic: Other Genetic Screening strategies for Virus-Host Interactions: RNAi and Haploid Screening 3. Lecturer: Kuo-Ming Lee
16	1. Physical course 2. Topic: Final Presentation and Group Discussion 3. Lecturer: Kuo-Ming Lee

先修課程

本課程沒有先修課程規定

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