移到主要內容
生物醫學研究所

生物醫學研究所
:::
蕭永晉

蕭永晉 / Yung-Chin Hsiao

職稱： 助理教授

現職： 長庚大學

信箱： hschin@mail.cgu.edu.tw

電話： 03-211-8800 ext. 3552

學歷： 中山醫學大學博士

專長領域： 癌症生物學 生物標誌 (多)體學或系統生物學 生物化學 醫學生物技術

個人網頁： https://pure.lib.cgu.edu.tw/zh/persons/yung-chin-hsiao-2

研究方向及研究室特色：

聚焦於運用蛋白體學/質譜學定量分析技術，系統性探討癌症相關生物標記(biomarker)、腫瘤抗原(tumor antigen)與腫瘤免疫機制，並致力於將研究成果轉譯至臨床醫學應用，發展可應用於分子診斷與標靶/免疫治療的分析平台。以「分析工具開發—分子機制理解—臨床問題導向」，將蛋白體學、質譜學、生物資訊與人工智慧整合為長期發展的研究平台。近年研究方向：

1.      體液與組織檢體之癌症生物標記探索與驗證。聚焦口腔癌，透過多重監測質譜定量技術，建立低含量蛋白質的精準定量流程，並結合抗胜肽單株抗體以提升靈敏度與專一性。涵蓋個體差異、日常生理變動與不同體液間的比較，逐步建構具臨床參考價值的定量基準，證實唾液作為非侵入性檢體的應用潛力。

2.      免疫多肽體(immunopeptidome)研究策略，發展腫瘤免疫與腫瘤抗原研究。結合蛋白體、基因體等多體學資料，解析腫瘤細胞表面呈現的抗原胜肽、分泌蛋白質與免疫微環境之間的交互作用。探討免疫熱/冷腫瘤特徵與腫瘤惡化機制，以及免疫治療反應與患者分層上的意義，為精準治療策略建立分子基礎。

3.      關鍵技術與分析平台的持續開發，包括多重標靶定量蛋白體分析流程的系統化設計，以及核酸適體(aptamer)篩選與最佳化。導入人工智慧與生物資訊方法，發展質譜層析圖譜的自動化與標準化分析工具，提升資料處理效率、降低人為偏差，並強化臨床應用的可行性。同時結合結構模擬與序列設計，擴展核酸適體於分子檢測與標靶治療上的應用。

最近五年所發表論文：

  1.  Wu CS, Li HP, Hsieh CH, Lin YT, Yi-Feng Chang I, Chung AK, Huang Y, Ueng SH, Hsiao YC, Chien KY, Luo JD, Chen CH, Liao WC, Hung JL, Yuan SN, OuYang CN, Chiang WF, Chien CY, Chuang HC, Chu LJ, Liu H, Yang CY, Robles AI, Rodriguez H, Lin HH, Yang HY, Hsueh C, Chang KP, Yu JS, and Chang YS. (2025) Integrated multi-omics analyses of oral squamous cell carcinoma reveal precision patient stratification and personalized treatment strategies. Cancer Lett 614, 217482.

    2. Liu CC, Hsiao YC, Lai WJ, Chiou CC, Chu LJ, Lin YT, Liu JC, and Yu JS. (2024) Development and optimization of a DNA aptamer to delay beta-bungarotoxin-induced lethality in a rodent model. Int J Biol Macromol 270 (Pt 2), 132240.

    3. Lee CC, Lin YC, Pan TY, Yang CH, Li PH, Chen SY, Gao JJ, Yang C, Chu LJ, Huang PJ, Yeh YM, Tang P, Chang YS, Yu JS, and Hsiao YC. (2023) HeapMS: An Automatic Peak-Picking Pipeline for Targeted Proteomic Data Powered by 2D Heatmap Transformation and Convolutional Neural Networks. Anal Chem 95 (42), 15486–15496.

    4. Hsiao YC, Chi LM, Chien KY, Chiang WF, Chen SF, Chuang YN, Lin SY, Wu CC, Chang YT, Chu LJ, Chen YT, Chia SL, Chien CY, Chang KP, Chang YS, and Yu JS. (2017) Development of a Multiplexed Assay for Oral Cancer Candidate Biomarkers Using Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry. Mol Cell Proteomics 16 (10), 1829–1849.

    5. Yu JS, Chen YT, Chiang WF, Hsiao YC, Chu LJ, See LC, Wu CS, Tu HT, Chen HW, Chen CC, Liao WC, Chang YT, Wu CC, Lin CY, Liu SY, Chiou ST, Chia SL, Chang KP, Chien CY, Chang SW, Chang CJ, Young JD, Pao CC, Chang YS, and Hartwell LH. (2016) Saliva protein biomarkers to detect oral squamous cell carcinoma in a high-risk population in Taiwan. Proc Natl Acad Sci U S A 113 (41), 11549–11554.

    6. Liu CC, Yang YH, Hsiao YC, Wang PJ, Liu JC, Liu CH, Hsieh WC, Lin CC, and Yu JS. Rapid and Efficient Enrichment of Snake Venoms from Human Plasma Using a Strong Cation Exchange Tip Column to Improve Snakebite Diagnosis. Toxins (Basel) 2021, 13 (2).

    7. Liu CC, Wu CJ, Hsiao YC, Yang YH, Liu KL, Huang GJ, Hsieh CH, Chen CK, and Liaw GW. Snake venom proteome of Protobothrops mucrosquamatus in Taiwan: Delaying venom-induced lethality in a rodent model by inhibition of phospholipase A2 activity with varespladib. J Proteomics 2020, 234, 104084.

    8. Lin YH, Wu CC, Su WT, Tseng PC, Hsueh YY, Hsiao YC, Chang KP, Yu JS, and Chuang YJ. Target peptide enrichment microfluidic chip for rapid detection of oral squamous cell carcinoma using stable isotope standards and capture by anti-peptide antibodies. Sensors and Actuators B: Chemical 2020, 322, 128607.

    9. Hsiao YC, Lin SY, Chien KY, Chen SF, Wu CC, Chang YT, Chi LM, Chu LJ, Chiang WF, Chien CY, Chang KP, Chang YS, and Yu JS. An immuno-MALDI mass spectrometry assay for the oral cancer biomarker, matrix metalloproteinase-1, in dried saliva spot samples. Anal Chim Acta 2020, 1100, 118-130.

    10. Chi LM#, Hsiao YC#, Chien KY#, Chen SF, Chuang YN, Lin SY, Wang WS, Chang IY, Yang C, Chu LJ, Chiang WF, Chien CY, Chang YS, Chang KP, and Yu JS. Assessment of candidate biomarkers in paired saliva and plasma samples from oral cancer patients by targeted mass spectrometry. J Proteomics 2020, 211, 103571.

    11. Liu CC, Yu JS, Wang PJ, Hsiao YC, Liu CH, Chen YC, Lai PF, Hsu CP, Fann WC, and Lin CC. Development of sandwich ELISA and lateral flow strip assays for diagnosing clinically significant snakebite in Taiwan. PLoS Negl Trop Dis 2018, 12 (12), e0007014.

    12. Liu CC, Lin CC, Hsiao YC, Wang PJ, and Yu JS. Proteomic characterization of six Taiwanese snake venoms: Identification of species-specific proteins and development of a SISCAPA-MRM assay for cobra venom factors. J Proteomics 2018, 187, 59-68.

    13. Hsiao YC, Chu LJ, Chen YT, Chi LM, Chien KY, Chiang WF, Chang YT, Chen SF, Wang WS, Chuang YN, Lin SY, Chien CY, Chang KP, Chang YS, and Yu JS. Variability Assessment of 90 Salivary Proteins in Intraday and Interday Samples from Healthy Donors by Multiple Reaction Monitoring-Mass Spectrometry. Proteomics Clin Appl 2018, 12 (2).

    14. Hsiao YC, Chu LJ, Chen JT, Yeh TS, and Yu JS. Proteomic profiling of the cancer cell secretome: informing clinical research. Expert Rev Proteomics 2017, 14 (9), 737-756.

    15. Hsiao YC, Chi LM, Chien KY, Chiang WF, Chen SF, Chuang YN, Lin SY, Wu CC, Chang YT, Chu LJ, Chen YT, Chia SL, Chien CY, Chang KP, Chang YS, and Yu JS. Development of a Multiplexed Assay for Oral Cancer Candidate Biomarkers Using Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry. Mol Cell Proteomics 2017, 16 (10), 1829-1849.

    16. Yu JS#, Chen YT#, Chiang WF#, Hsiao YC#, Chu LJ#, See LC, Wu CS, Tu HT, Chen HW, Chen CC, Liao WC, Chang YT, Wu CC, Lin CY, Liu SY, Chiou ST, Chia SL, Chang KP, Chien CY, Chang SW, Chang CJ, Young JD, Pao CC, Chang YS, and Hartwell LH. Saliva protein biomarkers to detect oral squamous cell carcinoma in a high-risk population in Taiwan. Proc Natl Acad Sci U S A 2016, 113 (41), 11549-11554.

    17. Chen H#, Hsiao YC#, Chiang SF, Wu CC, Lin YT, Liu H, Zhao H, Chen JS, Chang YS, and Yu JS. Quantitative analysis of wild-type and V600E mutant BRAF proteins in colorectal carcinoma using immunoenrichment and targeted mass spectrometry. Anal Chim Acta 2016, 933, 144-155.