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生物醫學研究所

生物醫學研究所
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郭敏玲

郭敏玲 / Ming-Ling Kuo

職稱： 教授

現職： 長庚大學生物醫學研究所所長

信箱： mingling@mail.cgu.edu.tw

電話： 3319/3320

學歷： 美國羅格斯大學博士

專長領域： 免疫學

個人網頁： https://sites.google.com/site/mlkmolecularimmunology/?pli=1&authuser=0

研究方向及研究室特色

本實驗室以過敏相關疾病為研究主軸，研究疾病的分子機制及開發新療法。過敏與氣喘疾在已開發國家盛行率愈來愈高，病嚴重影響人民的健康與消耗許多醫療資源，這類疾病是由多個遺傳與環境因子引起體內免疫系統不平衡反應所致，我們的研究重心主要在於深入探討可以調控過敏病的分子與細胞間反應，期許應用於研發新的防治方式。

氣喘病之基因治療：基於氣喘常為第二型T 輔助細胞過度反應所致，嚐試利用病毒載體攜帶抗發炎分子或第一型T 輔助細胞相關之細胞激素基因，經由氣道施予疾病模式小鼠，用以平衡過度活化的第二型T 輔助細胞免疫反應；或是攜帶可干擾第二型T 輔助細胞反應的基因片段，評估其抑制氣喘疾病各項指標的可行性。

藥物或是抗體引發T 調節細胞的發展：近年，如何調節過度活化的免疫反應成為免疫學的熱門課題，我們亦著手研究與表觀遺傳學的基因表達調控相關藥物、或是可改變T 輔助細胞族群活化功能的特殊抗體等，對人類周邊血球中的被激活T 輔助細胞或是氣喘疾病模式小鼠的免疫調節功能。在逐步研究這些藥物如何調控氣喘疾病發生的分子機制過程中，可以進一步發展運用這些藥物或抗體來治療氣喘疾病。

探討可治療氣喘中藥之科學證據：以臨床使用療效佳之方劑如「定喘湯」，利用氣喘動物模式來研究中藥使用之劑量、給藥時機、療效機制及安全性，做為分離出有效成分之基礎；也和中、西醫臨床醫師合作，完成療效評估。在中醫藥科學化方面，則將注重中藥作用於細胞訊號傳遞系統之機制，並搭配以中草藥材中的純化藥物，來探討並分析各藥物對周邊淋巴球細胞活性的調控或是對氣喘疾病模式小鼠的抑制發炎功能。

臍帶血淋巴細胞活性研究：比起骨髓，移植臍帶血中造血系統，有材料取得的方便性與排斥性低的優點。所以本實驗室的另外一個研究主題，是和長庚兒童醫院醫師合作，探討以不同細胞激素組合對臍帶血中 T 輔助細胞及自然殺手細胞之複製、死亡及活性的調控，或是評估不同免疫抑制劑對細胞功能的影響，可以對臍帶血移植時的免疫反應，有更多的了解。

論文與著作

Original articles:

 

1.      Chang K-W, Chen J-T, Chiang-Ni C, Dang Thi Thanh Thao, Huang Y-T, Wu H-Y, Kuo M-L, Kao K-C, Chiu C-H, Lai C-H*. 2025 (Oct 5). Microplastic exposure aggravates pneumococcus-induced inflammation in macrophages by activating ferroptosis. J. Hazard. Mater. 2025; 497: 139696-139709. (DOI: 10.1016/j.jhazmat.2025.139696.)

2.      Ma J, Yang T-H, Hsiung C-C, Sun H-Y, Kuo M-L*. 2025 (Jun 19). Isolate circulating mesenchymal stromal cells using a gradient-free and growth factor-free method. Stem Cells Int. 2025; 5545892-5545903. (DOI: 10.1155/sci/5545892)

3.      Lu C-H, Ma J, Lin M-C, Wu C-J, Kuo C-Y, Chiang-Ni C, Kuo M-L*. 2025 (May 22). Streptococcal pyrogenic exotoxin a induces regulatory T cells via TNF-α-TNFR2 signaling. Med. Microbiol. Immunol. 2025; 214(1): 25-35. (DOI: 10.1007/s00430-025-00835-1)

4.      Kuo Y-S, Chiang P-C, Kuo C-Y, Huang C-G, Kuo M-L, Chiu Y-F. 2025 (Jun). Inhibition of influenza A virus proliferation in mice via universal RNA interference. Antivir. Res. 2025; 238: 106149-106160. (DOI: 10.1016/j.antiviral.2025.106149)

5.      Chen J-C, Hsu M-H, Kuo R-L, Wang L-T, Kuo M-L, Tseng L- Y, Chang H-L, Chiu C-H*. 2025 (Mar 11). mRNA-127 1 3 is placenta-permeable and immunogenic in the fetus. Mol. Ther. Nucleic. Acids. 2025; 36(1): 102489-102501. (DOI: 10.1016/j.omtn.2025.102489)

6.      Kuo C-Y, Huang C-Y, Chen H-M, Chen L-C, Kuo M-L*. 2025 (Feb 20). Antagonism of CD28 blocks allergic responses in the ovalbumin-induced asthmatic model mice. Int. Immunopharmacol. 2025; 148: 114071-114082. (DOI:10.1016/j.intimp.2025.114071)

7.      Ma J, Chiu Y-F, Kao C-C, Chun-Ning C, Chen C-Y, Lai C-H, Kuo M-L*.2024 (Sep 4). Fine particulate matter manipulates immune response to exacerbate microbial pathogenesis in the respiratory tract. Eur. Respir. Rev. 2024; 33(173): 230259-230272. (DOI: 10.1183/16000617.0259-2023)

8.      Chang-Chien J, Huang J-L, Tsai H-J, Wang S-L, Kuo M-L‡, Yao T-C‡.2024 (Aug 22). Vitamin D ameliorates particulate matter induced mitochondrial damages and calcium dyshomeostasis in BEAS-2B human bronchial epithelial cells. Respir. Res. 2024; 25(1): 321-335. (DOI: 10.1186/s12931-024-02951-7)

9.      Chang-Chien J, Kuo M-L, Tseng Y-L, Huang H-Y, Tsai H-J, Yao T-C. 2024 (Aug 1). Differential effects of long- and short-term exposure to PM2.5 on accelerating telomere shortening: from in vitro to epidemiological studies. Ecotox. Environ. Safe. 2024; 281: 116650. (DOI: 10.1016/j.ecoenv.2024.116650. Epub 2024 Jul 3.)

10.  Chen J-C*, Ou L-S, Kuo M-L, Tseng L-Y, Chang H-L. 2024 (June 8). Autoantigen exposure in murine fetuses elicited nonpathogenic autoimmunity. Arch. Med. Res. 2024; 55(5): 103013-103022. (DOI: 10.1016/j.arcmed.2024.103013)

11.  Huang Y-A, Chen J-C Chiang P-C, Chen L-C, Kuo M-L #. 2023 (Nov 15). Adeno-associated viral vector-delivered pannexin-1 mimetic peptide alleviates airway inflammation in an allergen-sensitized mouse model. Hum. Gene Ther. 2023; 34 (21-22): 1107-1117. (DOI: 10.1089/hum.2023.078)

12.  Chiang P-C, Chen J-C, Chen L-C, Kuo M-L*. 2022 (Aug 16). Adeno-associated virus-mediated interleukin-12 gene expression alleviates lung inflammation and type 2 T-helper-responses in ovalbumin-sensitized asthmatic mice. Hum. Gene Ther2022; 33 (19-20): 1052-1061. (DOI: 10.1089/hum.2022.034)

13.  Ting N-C, Chen Y-H, Chen J-C, Huang W-C, Liou C-J, Chen L-C, Yang S-H‡, Kuo M-L‡. 2022 (Jun 30). Perilla fruit water extract attenuates inflammatory responses and alleviates neutrophil recruitment via MAPK/JNK-AP-1/c-Fos signaling pathway in ARDS animal model. Evid. Based Complement. Alternat. Med. 2022; (23): 4444513-444525. (DOI: 10.1155/2022/4444513)

14.  Hsu C-Y, Chueh Y-S, Kuo M-L, Lee P-T, Hsiao H-S, Huang J-L, Lin S-J. 2021 (Dec 22). Expansion of invariant natural killer T cells from systemic lupus erythematosus patients by alpha-Galactosylceramide and IL-15. PLoS One. 2021; 16(12): e0261727. (DOI: 10.1371/journal.pone.0261727)

15.  Chen L-C, Tseng H-M, Kuo M-L, Chiu C-Y, Liao S-L, Su K-W, Tsai M-H, Hua M-C, Lai S-H, Yao T-S, Yeh K-W, Wu A-H, Yu H-Y, Huang J-L, Huang S-K. 2021 (Nov). Levels of 15-HETE and TXB in exhaled breadth condensates as markers for diagnosis of childhood asthma and its therapeutic outcome. Pediatr. Allergy Immunol. 2021; 32(8): 1673-1680.
(DOI: 10.1111/pai.13587.)

16.  Ma J, Liu M-X, Chen L-C, Shen J-J, Kuo M-L* 2021 (Sep 21). Ding Chuan Tang attenuates airway inflammation and eosinophil infiltration in ovalbumin-sensitized asthmatic mice. Biomed Res. Int. 2021; 20: 6692772. (DOI: 10.1155/2021/6692772) 2

17.  Chang-Chien J, Huang J-L, Tsai H-J, Wang S-L, Kuo M-L‡. Yao T-C‡. 2021 (Oct). Particulate matter causes telomere shortening and cellular senescence in human lung epithelial cells. Ecotox. Environ. Safe. 2021; 222: 112484-112492. (DOI: 10.1016/j.ecoenv.2021.112484)

18.  Chen J-C, Chan C-C, Ting N-C, Kuo M-L*. 2021 (June). Allergen exposure in murine neonates promoted the development of allergic lungs. Biomedicines. 2021; 9(6): 688-703.
(DOI: 10.3390/biomedicines9060688)

19.  Huang Y-A, Chen J-C, Wu C-C, Hsu C-W, Ko Albert M-S, Chen L-C, Kuo M-L*. 2021 (June). Reducing lung ATP levels and alleviating asthmatic airway inflammation through adeno-associated viral vector-mediated CD39 expression. Biomedicines. 2021; 9(6): 656-669.(DOI: 10.3390/biomedicines9060656)

20.  Chen J-C, Ou L-S, Kuo M-L, Tseng L-Y, Chang H-L, Chen S-C, Chui C-H. 2021 (March). Fetal macrophages exposed to Salmonella antigens elicit protective immunity against overwhelming Salmonella challenge in a murine model. Biomedicines. 2021. 9(3): 245-260. (DOI: 10.3390/biomedicines9030245)

 

Review articles:

1.          Lai Y-R, Chang Y-F, Ma J, Chiu C-H, Kuo M-L‡, Lai C-H. 2021 (Nov 15). From DNA damage to cancer progression: potential effects of cytolethal distending toxin. Front. Immunol. 2021; 12:760451-760459.
(DOI: 10.3389/fimmu.2021.760451)

2.          Hsu L-J, Liu C-L, Kuo M-L, Shen C-N, Shen C-R. 2021 (Oct). An alternative cell therapy for cancers: Induced pluripotent stem cell (iPSC)-derived natural killer cells. Biomedicines. 2021; 9(10): 1323-1339. (DOI: 10.3390/biomedicines9101323)

 

Book chapters:

1.          Lin S-J, Lee Y-C, Yan D-C, Kuo M-L. Natural killer cells in allergy. Ch38, pp 511-517, in Lotze M and Thomson A, eds. Natural Killer Cells, Basic Science and Clinical Application, Elsevier, San Diego, CA, USA. 2009.

2.          Lin S-J, Lee P-T, Kuo M-L. Cytokine activation of natural killer cells. Ch19. pp in Cancer Vaccines: Methods and Protocols, Methods in Molecular Biology, Vol 1139. Springer Science+Business Media, New York, NY, USA. 2014. DOI: 10.1007/978-1-4939-0345-0_19.

 

專書、教科書(Special books, textbooks):

1.          郭敏玲, 林錫賢, 黃永安, 吳宇恆. 2024. 免疫學( Coico：Immunology A Short Course 8/E)。 新北，台灣，ISBN 978-986-378-41-1，600頁， https://www.gau-lih.com.tw/tier/front/bin/ptdetail.phtml?Part=0704A8&Category=0。