生物醫學研究所

研究方向及研究室特色：

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長庚大學陳怡婷老師實驗室以生物質譜技術開發及分析化學為基礎，發展生物分子之分離技術與生物質譜檢測及定量方法；與泌尿科、腎臟科、婦產科、小兒科等臨床醫生合作，運用分析化學、生物質譜技術、蛋白質體及代謝體等後基因體學研究方法，全面性的研究正常與疾病生物系統(細胞株及臨床收集的體液或組織)中蛋白質與代謝物之群體變化；臨床醫學應用方面，透過與國內及國外等機構，我們研究膀胱癌、腎臟癌、及腎衰竭病人的尿液、口腔癌症組織及口水、及婦產科羊水的蛋白質體學及代謝體學；並且在長庚大學的蛋白質體核心實驗室裡，負責標靶蛋白質質譜定量技術及穩定同位素化學標定代謝體技術平台的開發與維護，希望將實驗室的研發成果轉譯成為臨床醫生可以使用的診療工具，監測大小重要生物分子在疾病所扮演的角色，以期望朝向臨床應用及個人化醫療邁進。

最近五年所發表論文：

1.  Jau-Song Yu, Yi-Ting Chen, Wei-Fang Chiang, Yung-Chin Hsiao, Lichieh Julie Chu, Lai-Chu See, Chi-Sheng Wu, Hui-Tzu Tu, Hsiao-Wei Chen, Chia-Chun Chen, Wei-Chao Liao, Ya-Ting Chang, Chih-Ching Wu, Che-Yi Lin, Shyun-Yeu Liu, Shu-Ti Chiou, Shu-Li Cha, Kai-Ping Chang, Chih-Yen Chien, Chee-Jen Chang, John D. Young, Chia C. Pao, Yu-Sun Chang*, Leland H. Hartwell*. Saliva protein biomarkers to detect oral squamous cell carcinoma in a high-risk population in Taiwan. PNAS. 2016;113:11549–11554.
   
   2. Ya-Ju Hsieh, Kun-Yi Chien, Chun-Ming Chang, Cheng-Yu Hung, Liang Li, Wei-Fan Chiang, Cheng-Chia Lee, Chih-Hsiang Chang, Ying-Hsu Chang, Jau-Song Yu*, Yi-Ting Chen*. Development of a Method for Dansylation of Metabolites Using Organic Solvent-Compatible Buffer Systems for Amine/Phenol Submetabolome Analysis. Analytica Chimica Acta. 2022;1189:339218.
   
   3. Yi-Ting Chen, Wan-Rou Liao, Hsueh-Ting Wang, Hsiao-Wei Chen, Sung-Fang Chen*. Targeted Protein Quantitation in Human Body Fluids by Mass Spectrometry. Mass Spectrom Rev. 2023;42(6):2379-2403. doi: 10.1002/mas.21788.
   
   4. Ya-Ju Hsieh*, Cheng-Yu Hung, Yi-Ting Chen, Yu-Tsun Lin, Kai-Ping Chang, Wei-Fan Chiang, Chih-Yen Chien, Chih-Ching Wu, Liang Li, Jau-Song Yu, Kun-Yi Chien*. Enhanced Nano-LC-MS for Analyzing Dansylated Oral Cancer Tissue Metabolome Dissolved in Solvents with High Elution Strength. Analytica Chimica Acta. 2025;1337:343514. doi: 10.1016/j.aca.2024.343514.
   
   5. Ya-Ju Hsieh, Yao-Lung Chang*, Yi-Hsin Wu, Cheng-Yu Hung, Liang Li, Jau-Song Yu, Chih-Hsiang Chang, Wei-Ju Tu, Yi-Ting Chen*. Metabolic profiling of amniotic fluid by differential 12C-/13C-isotope dansylation labeling LC-MS for application in trisomy 21 fetuses. Metabolomics. 2025 Aug 11;21(5):106.
   
   6. Yi-Ting Chen*, Hsiao-Wei Chen, Chun-Feng Wu, Lichieh Julie Chu, Wei-Fang Chiang, Chih-Ching Wu, Jau-Song Yu, Cheng-Han Tsai, Kung-Hao Liang, Yu-Sun Chang, Maureen Wu, Wei-Ting Ou Yang. Development of a multiplexed LC-MRM/MS method for evaluation of salivary proteins as oral cancer biomarkers. Mol Cell Proteomics. 2017;16:799-811.
   
   7. Chi-Hung Shao, Chien-Lun Chen, Chao-Jung Chen, Shu-Hsuan Fu, Yi-Ting Chen, Yu-Sun Chang, Jau-Song Yu, Chiun-Gung Juo*, Kun-Pin Wu*. Metabolite marker discovery for the detection of bladder cancer by comparative metabolomics. Oncotarget. 2017 Jun 13;8(24):38802-38810.
   
   8. Yung-Chin Hsiao, Lang-Ming Chi, Kun-Yi Chien, Wei-Fan Chiang, Szu-Fan Chen, Yao-Ning Chuang, Shih-Yu Lin, Chia-Chun Wu, Ya-Ting Chang, Lichieh Julie Chu, Yi-Ting Chen, Shu-Li Chia, Chih-Yen Chien, Kai-Ping Chang, Yu-Sun Chang, Jau-Song Yu. Development of a multiplexed assay for oral cancer candidate biomarkers using peptide immunoaffinity enrichment and targeted mass spectrometry. Mol Cell Proteomics. 2017 Oct;16(10):1829-1849.
   
   9. Cheng-Han Tsai, Yi-Ting Chen*. Clinical proteomics of genitourinary cancer for the development of biomarkers. Clinics in Oncology. 2017;2:1326-1327.
   
   10. Ting-Wen Chen, Chi-Ching Lee, Hsuan Liu, Chi-Sheng Wu, Curtis R. Pickering, Po-Jung Huang, Jing Wang, Ian Yi-Feng Chang, Yuan-Ming Yeh, Chih-De Chen, Hsin-Pai Li, Ji-Dung Luo, Bertrand Chin-Ming Tan, Timothy En Haw Chan, Chuen Hsueh, Lichieh Julie Chu, Yi-Ting Chen, Bing Zhang, Chia-Yu Yang, Chih-Ching Wu, Chia-Wei Hsu, Lai-Chu See, Petrus Tang, Jau-Song Yu, Wei-Chao Liao, Wei-Fan Chiang, Henry Rodriguez, Jeffrey N. Myers, Kai-Ping Chang*, Yu-Sun Chang*. APOBEC3A is an oral cancer prognostic biomarker in Taiwanese carriers of an APOBEC deletion polymorphism. Nature Communications. 2017;8:465.
   
   11. Yung-Chin Hsiao, Lichieh Julie Chu, Yi-Ting Chen, Lang-Ming Chi, Kun-Yi Chien, Wei-Fan Chiang, Ya-Ting Chang, Szu-Fan Chen, Wei-Shun Wang, Yao-Ning Chuang, Shih-Yu Lin, Chih-Yen Chien, Kai-Ping Chang, Yu-Sun Chang, Jau-Song Yu. Variability assessment of 90 salivary proteins in intra-day and inter-day samples from healthy donors by multiple reaction monitoring-mass spectrometry. Proteomics Clinical Applications. 2018 Mar;12(2). doi: 10.1002/prca.201700039.
   
   12. Cheng-Han Tsai, Yi-Ting Chen*, Ying-Hsu Chang, Chuen Hsueh, Chung-Yi Liu, Yu-Sun Chang, Chien-Lun Chen*, Jau-Song Yu*. Systematic Verification of Bladder Cancer-Associated Tissue Protein Biomarker Candidates in Clinical Urine Specimens. Oncotarget. 2018;9:30731-30747.
   
   13. Cheng-Chia Lee, Chih-Hsiang Chang, Shao-Wei Chen*, Pei-Chun Fan, SuWei Chang, Yi-Ting Chen, Yu-Yun Nan, Pyng-Jing Lin, Feng-Chun Tsai. Preoperative Risk Assessment Improves Biomarker Detection for Predicting Acute Kidney Injury After Cardiac Surgery. PLOS ONE. 2018;13(9):e0203447.
   
   14. Wei-Cheng Lin, Archan Chakraborty, Shih-Chia Huang, Pei-Yu Wang, Ya-Ju Hsieh, Kun-Yi Chien, Yen-Hsien Lee, Chia-Chun Chang, Hsiang-Yu Tang, Yu-Tsun Lin, Chang-Shung Tung, Ji-Dung Luo, Ting-Wen Chen, Tzu-Yang Lin, Mei-Ling Cheng, Yi-Ting Chen, Chau-Ting Yeh, Ji-Long Liu, Li-Ying Sung, Ming-Shi Shiao, Jau-Song Yu, Yu-Sun Chang, Li-Mei Pai*. Histidine-dependent protein methylation is required for compartmentalization of CTP Synthase. Cell Reports. 2018;24:2733–2745.
   
   15. Cheng-Chia Lee, Ya-Ju Hsieh, Shao-Wei Chen, Shu-Hsuan Fu, Chia-Wei Hsu, Chih-Ching Wu, Wei Han, Yunong Li, Huan Tao, Yu-Sun Chang, Jau-Song Yu, Liang Li, Chih-Hsiang Chang, Yi-Ting Chen*. Bretschneider solution-induced alterations in the urine metabolome in cardiac surgery patient. Scientific Reports. 2018;8:17774.
   
   16. Yi-Ting Chen*, Cheng-Han Tsai, Chien-Lun Chen, Jau-Song Yu, Ying-Hsu Chang*. Development of Biomarkers of Genitourinary Cancer using Mass Spectrometry-Based Clinical Proteomics. Journal of Food and Drug Analysis. 2019;27(2):387-403.
   
   17. Yi-Ting Chen*, Hsin-Chien Huang, Ya-Ju Hsieh, Shu-Hsuan Fu, Liang Li, Chien-Lun Chen, Lichieh Julie Chu, Jau-Song Yu. Targeting amine- and phenol-containing metabolites in urine by dansylation isotope labeling and liquid chromatography mass spectrometry for evaluation of bladder cancer biomarkers. Journal of Food and Drug Analysis. 2019;27(2):460-474.
   
   18. Chia-Wei Hsu, Yi-Ting Chen, Ya-Ju Hsieh, Kai-Ping Chang, Pei-Chun Hsueh, Ting-Wen Chen, Jau-Song Yu, Yu-Sun Chang, Liang Li*, Chih-Ching Wu*. Integrated analyses utilizing metabolomics and transcriptomics reveal perturbation of the polyamine pathway in oral cavity squamous cell carcinoma. Analytica Chimica Acta. 2019;1050:113-122.
   
   19. Jia-Jin Chen, Pei-Chun Fan, George Kou, Su-Wei Chang, Yi-Ting Chen, Cheng-Chia Lee, Chih-Hsiang Chang*. Meta-analysis: urinary calprotectin for discrimination of intrinsic and prerenal acute kidney injury. J Clin Med. 2019;8(1):E74.
   
   20. Pei-Chun Fan, Jason Chih-Hsiang Chang, Chia-Ni Lin, Cheng-Chia Lee, Yi-Ting Chen, Pao-Hsien Chu, George Kou, Yueh-An Lu, Chih-Wei Yang, Yung-Chang Chen*. Serum Indoxyl Sulfate Predicts Adverse Cardiovascular Events in Patients with Chronic Kidney Disease. Journal of the Formosan Medical Association. 2019;118:1099-1106.
   
   21. Hao-Wei Chu, Kai-Ping Chang, Chia-Wei Hsu, Ian Yi-Feng Chang, Hao-Ping Liu, Yi-Ting Chen, Chih-Ching Wu. Identification of salivary biomarkers for oral cancer detection with untargeted and targeted quantitative proteomics approaches. Molecular & Cellular Proteomics. 2019;18(9):1796-1806.
   
   22. Yi-Ting Chen*, Wei-Ting Ou Yang, Horng-Heng Juang, Chien-Lun Chen*, Hsiao-Wei Chen, Ke-Hung Tsui, Ying-Hsu Chang, Cheng-Han Tsai, Chuen Hsueh, Wei-Chao Liao. Proteomic characterization of arsenic and cadmium exposure in bladder cells. Rapid Commun Mass Spectrom. 2020;34(S1):e8578.
   
   23. Cheng-Chia Lee, Chih-Hsiang Chang, Ya-Lien Cheng, George Kuo, Shao-Wei Chen, Yi-Jung Li, Yi-Ting Chen, Ya-Chung Tian*. Diagnostic performance of Cyclophilin A in cardiac surgery-associated acute kidney injury. J Clin Med. 2020;9:108. doi: 10.3390/jcm9010108.
   
   24. Pei-Chun Hsueh, Kuo-An Wu, Chia-Yu Yang, Chia-Wei Hsu, Chih-Liang Wang, Chu-Mi Hung, Yi-Ting Chen, Jau-Song Yu, Chih-Ching Wu*. Metabolomic profiling of parapneumonic effusion reveals a regulatory role of dipeptides in interleukin-8 production in neutrophil-like cells. Analytica Chimica Acta. 2020;1128:238-250.
   
   25. Jo-Chuan Liu, Yi-Ting Chen, Ya-Ju Hsieh, Chia-Chun Wu, Ming-Chyi Huang, Yu-Chao Hsu, Chun-Te Wu, Chih-Ken Chen, Srinivas Dash, Jau-Song Yu*. Association of urinary ketamine and APOA1 levels with bladder dysfunction in ketamine abusers revealed via proteomics and targeted metabolite analyses. Scientific Reports. 2021;11:9583.
   
   26. Jia-You Lin, Bao-Rong Juo, Yu-Hsuan Yeh, Shu-Hsuan Fu, Yi-Ting Chen, Chien-Lun Chen*, Kun-Pin Wu*. Putative markers for the detection of early-stage bladder cancer selected by urine metabolomics. BMC Bioinformatics. 2021;22:305.