天然藥物碩士班

職稱： 專任教授 現職： 天然藥物碩士班專任教授 / Full-time Professor 信箱： liaoch@mail.cgu.edu.tw 電話： 分機5522 學歷： 台灣大學藥理所博士 專長領域： 天然物藥理藥效評估 、抗血栓與發炎藥理學 個人網頁： https://pure.lib.cgu.edu.tw/en/persons/hsiang-ruei-liao-2 研究室：天然物藥理研究室/Natural Products Pharmacology Laboratory 研究室成員： 碩士班研究生6人、專任研究助理2人

天然物在心血管疾病之機轉探討;針對冠狀動脈的發生過程與機制，研究藥物抑制血小板以及發炎細胞（嗜中性白血球）活化的相關機轉，藉以研發對於心血管疾病的有效用藥。研究過程中針對藥物對血小板以及嗜中性白血球細胞內訊息傳遞之探討，包括藥物與細胞表面受體結合、酵素活性、自由基清除、細胞內鈣離子變化，以及細胞內二級訊息傳遞之分析。 探討天然藥物抗自由基之研究；自由基參與許多生理及病理現象，包括免疫反應、老化、甚至與癌症的發生亦有關連。自由基除了被白血球用於殺菌外，不正常過多的自由基對許多細胞造成不必要的傷害。因此藉由中草藥對自由基清除的研究，對於中草藥在藥品或健康食品發展上，能有更進一步的幫助。 研究血液中細胞與細胞間的交互作用；探討細胞表面吸附分子生理及病理角色，並且配合天然藥物對細胞間交互作用的影響，探討吸附分子表現之訊息傳遞，以期瞭解吸附分子在血栓形成過程中，生理病理角色，並且藉由藥物機制的探討，找出能預防血栓形成更有效的藥物。研究方法以全血的狀態下進行，目的是可以達到較接近生理狀態。 研究天然藥物調控人類嗜中性白血球與血小板交互作用機轉、探討血小板刺激嗜中性白血球細胞外陷阱捕捉 (neutrophil extracellular traps; NET) 的機轉與藥物效果，並以動物血栓及肝臟再灌流模式，評估天然藥物的應用。

姓名 學位 論 文 題 目 陳琛龍 碩士 探討蘭嶼肉豆蔻果實萃取物 MCF-32 抑制人類嗜中性白血球產生超氧自由基之機轉 高禹軺 碩士 探討台灣大戟根部萃取物 larixol 抑制 fMLP 誘導人類嗜中性白血球產生超氧自由基之機轉 王思媛 碩士 牙香樹萃取物 3’-hydroxygenkwanin 抑制人類嗜中性白血球發炎機轉之探討 林彥均 碩士 化合物 BS-088 抑制 fMLP 誘導人類嗜中性白血球釋出超氧自由基之作用機轉 顏晨宇 碩士 探討化合物 BW-116 抑制人類嗜中性白血球釋出超氧自由基之機制 陳品宏 碩士 探討牙香樹萃取物 Velutin 抑制人類嗜中性白血球釋出超氧自由基之機制 曾姿綺 碩士 探討日本賽衛矛萃取物 NKSE-5A 與台灣大戟萃取物Larixol 抑制 fMLP 所誘導人類嗜中性白血球釋出超氧自由基之機轉 林芩萱 碩士 探討細本葡萄萃取物VT-1 抑制人類嗜中性白血球釋出超氧自由基之機制 潘群凱 碩士 探討長花厚殼樹萃取物 ELE-9 抑制人類嗜中性白血球釋出超氧自由基之機轉 郭俞廷 碩士 探討化合物 DJA1 抑制人類嗜中性白血球釋出超氧自由基之機制 簡鏡儒 碩士 探討厚朴萃取物 honokiol 抑制人類嗜中性白血球釋出超氧自由基之機轉 簡吟桓 碩士 探討田麻科植物西印度櫻桃葉部萃取物 Tectochrysin (MCL-1) 抑制人類嗜中性白血球釋出超氧自由基之機轉 林順治 碩士 探討芸香科植物山刈葉果實萃取物2-(1`-Geranyloxy)-4,6-Dihydroxyacetophenone (MSF-4) 誘導人類嗜中性白血球釋出超氧自由基之機轉 劉佳欣 碩士 探討山刈葉果實萃取物 Pachypodol 與化合物 ND700C 抑制 fMLP 所引發人類嗜中性白血球釋出超氧自由基之機轉 黃昱如 碩士 探討蘭嶼花椒葉部萃取物 N-methylflindersine (ZIL-25)與化合物 CMH-1001 抑制 fMLP 引發人類嗜中性白血球釋出超氧自由基之機轉 黃羿嘉 碩士 探討菲律賓胡椒萃取物 PP-6 與化合物 HCL-85 抑制人類嗜中性白血球釋出超氧自由基之機轉 王鎂盈 碩士 探討 cytochalasinB 加強 fMLP 誘發人類嗜中性白血球表現的機轉 蔡宜芳 碩士 YC-1 抑制脂多醣體刺激人類樹突細胞成熟過程的機制探討 劉秀真 碩士 F-6HQO 對於血管平滑肌細胞之抗增生機轉的探討:自由基的角色 陳俊廷 碩士 探討 F-7HQO 抑制血小板凝集之機制 邱惠雯 碩士 1.研究 CAPE 於人類嗜中性白血球中抗氧化機制 2.嗜中性白血球,血小板及血管平滑肌之蛋白質體學研究 許惟傑 碩士 探討 7-Hydroxy-Isoflavonoid 衍生物於體內及體外對血小板凝集之影響和可能機制 謝硯如 碩士 Celecoxib 對人類嗜中性白血球釋出超氧自由基機轉之探討

*, corresponding author Liao HR*, Kao YY, Leu YL, Liu FC, Tseng CP. Larixol inhibits fMLP-induced superoxide anion production and chemotaxis by targeting the βγ subunit of Gi-protein of fMLP receptor in human neutrophils. Biochem Pharmacol. 2022, 201:115091. (SCI) (IF: 6.100; R/C=46/279 in PHARMACOLOGY & PHARMACY) Liao HR*, Lin CH, Chen JJ, Liu FC, Tseng CP. The anti-inflammatory effect of ε-viniferin by specifically targeting formyl peptide receptor 1 on human neutrophils. Chem Biol Interact. 2021, 345:109490. (SCI) (IF: 5.168; R/C=73/279 in PHARMACOLOGY & PHARMACY) Chu YC, Chang CH, Liao HR, Fu SL, Chen JJ. Anti-Cancer and Anti-Inflammatory Activities of Three New Chromone Derivatives from the Marine-Derived Penicillium citrinum. Mar Drugs. 2021, 19(8):408. (SCI) (IF: 6.085; R/C=10/63 in CHEMISTRY, MEDICINAL) Tsai HJ, Cheng JC, Kao ML, Chiu HP, Chiang YH, Chen DP, Rau KM, Liao HR, Tseng CP. Integrin αIIbβ3 outside-in signaling activates human platelets through serine 24 phosphorylation of Disabled-2. Cell Biosci. 2021, 11(1):32. (SCI) (IF: 9.606; R/C=31/296 in BIOCHEMISTRY & MOLECULAR BIOLOGY) Chu YC, Chang CH, Liao HR, Cheng MJ, Wu MD, Fu SL, Chen JJ. Rare Chromone Derivatives from the Marine-Derived Penicillium citrinum with Anti-Cancer and Anti-Inflammatory Activities. Mar Drugs. 2021, 19(1):25. (SCI) (IF: 6.085; R/C=10/63 in CHEMISTRY, MEDICINAL) Tseng CP, Huang YL, Chang YW, Liao HR, Chen YL, Hsieh PW. Polysaccharide-containing fraction from Artemisia argyi inhibits tumor cell-induced platelet aggregation by blocking interaction of podoplanin with C-type lectin-like receptor 2. J Food Drug Anal. 2020, 28(1):115-123. (SCI) (IF: 6.157; R/C=45/279 in PHARMACOLOGY & PHARMACY). Wang SL, Liao HR, Cheng MJ, Shu CW, Chen CL, Chung MI, Chen JJ. Four New 2-(2-Phenylethyl)-4H-chromen-4-one Derivatives from the Resinous Wood of Aquilaria sinensis and Their Inhibitory Activities on Neutrophil Pro-Inflammatory Responses. Planta Med. 2018, 84(18):1340-1347. (SCI) (IF: 3.007; R/C=41/63 in CHEMISTRY, MEDICINAL) Liao HR, Chen IS, Liu FC, Lin SZ, Tseng CP. 2',3-dihydroxy-5-methoxybiphenyl suppresses fMLP-induced superoxide anion production and cathepsin G release by targeting the β-subunit of G-protein in human neutrophils. Eur J Pharmacol. 2018, 829:26-37. (SCI) (IF: 5.195; R/C=71/279 in PHARMACOLOGY & PHARMACY) Chang YW, Tseng CP, Lee CH, Hwang TL, Chen YL, Su MT, Chong KY, Lan YW, Wu CC, Chen KJ, Lu FH, Liao HR, Hsueh C, Hsieh PW. β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release. Am J Physiol Lung Cell Mol Physiol. 2018, 314(4):L654-L669. (SCI) (IF: 6.011; R/C=12/81 in PHYSIOLOGY) Tsai HJ, Chien KY, Liao HR, Shih MS, Lin YC, Chang YW, Cheng JC, Tseng CP. Functional links between Disabled-2 Ser723 phosphorylation and thrombin signaling in human platelets. J Thromb Haemost. 2017, 15(10):2029-2044. (SCI) (IF: 16.036; R/C=3/67 in PERIPHERAL VASCULAR DISEASE) Cheng LY, Liao HR, Chen LC, Wang SW, Kuo YH, Chung MI, Chen JJ. Naphthofuranone derivatives and other constituents from Pachira aquatica with inhibitory activity on superoxide anion generation by neutrophils. Fitoterapia. 2017, 117:16-21. (SCI) (IF: 3.204; R/C=166/279 in PHARMACOLOGY & PHARMACY) Chen JJ, Tsai TH, Liao HR, Chen LC, Kuo YH, Sung PJ, Chen CL, Wei CS. New Sesquiterpenoids and Anti-Platelet Aggregation Constituents from the Rhizomes of Curcuma zedoaria. Molecules. 2016, 21(10):1385. (SCI) (IF: 4.927; R/C=114/296 in BIOCHEMISTRY & MOLECULAR BIOLOGY) Liao HR, Chien CR, Chen JJ, Lee TY, Lin SZ, Tseng CP. The anti-inflammatory effect of 2-(4-hydroxy-3-prop-2-enyl-phenyl)-4-prop-2-enyl-phenol by targeting Lyn kinase in human neutrophils. Chem Biol Interact. 2015, 236:90-101. (SCI) (IF: 5.168; R/C=73/279 in PHARMACOLOGY & PHARMACY) Chang YW, Hsieh PW, Chang YT, Lu MH, Huang TF, Chong KY, Liao HR, Cheng JC, Tseng CP. Identification of a novel platelet antagonist that binds to CLEC-2 and suppresses podoplanin-induced platelet aggregation and cancer metastasis. Oncotarget. 2015, 6(40):42733-48. (SCI) (IF: 5.168; R/C=44/217 in ONCOLOGY) Tseng WL, Chen TH, Huang CC, Huang YH, Yeh CF, Tsai HJ, Lee HY, Kao CY, Lin SW, Liao HR, Cheng JC, Tseng CP. Impaired thrombin generation in Reelin-deficient mice: a potential role of plasma Reelin in hemostasis. J Thromb Haemost. 2014, 12(12):2054-64. (SCI) (IF: 16.036; R/C=3/67 in PERIPHERAL VASCULAR DISEASE) Kuo WL, Liao HR, Chen JJ. Biflavans, Flavonoids, and a Dihydrochalcone from the Stem Wood of Muntingia calabura and Their Inhibitory Activities on Neutrophil Pro-Inflammatory Responses. Molecules. 2014, 19(12):20521-20535. (SCI) (IF: 4.927; R/C=114/296 in BIOCHEMISTRY & MOLECULAR BIOLOGY) Chuang LP, Chen NH, Lin SW, Chang YL, Liao HR, Lin YS, Chao IJ, Lin Y, Pang JH. Increased C-C chemokine receptor 2 gene expression in monocytes of severe obstructive sleep apnea patients and under intermittent hypoxia. PLoS One. 2014, 9(11):e113304. (SCI) (IF: 3.752; R/C=29/73 in MULTIDISCIPLINARY SCIENCES) Liu FC, Liou JT, Liao HR, Mao CC, Yang P, Day YJ. The anti-aggregation effects of ondansetron on platelets involve IP3 signaling and MAP kinase pathway, but not 5-HT3-dependent pathway. Thromb Res. 2012, 130(3):e84-94. (SCI) (IF: 10.407; R/C=6/67 in PERIPHERAL VASCULAR DISEASE) Liu FC, Day YJ, Liou JT, Yu HP, Liao HR*. Splitomicin inhibits fMLP-induced superoxide anion production in human neutrophils by activate cAMP/PKA signaling inhibition of ERK pathway. Eur J Pharmacol, 2012, 688(1-3):68-75. (SCI) (IF: 5.195; R/C=71/279 in PHARMACOLOGY & PHARMACY) Liao HR*, Chen JJ, Chien YH, Lin SZ, Lin S, Tseng CP. 5-Hydroxy-7-methoxyflavone inhibits N-formyl-L-methionyl-L-leucyl- Lphenylalanine-induced superoxide anion production by specific modulate membrane localization of Tec with a PI3K independent mechanism in human neutrophils. Biochem Pharmacol. 2012, 84(2):182-91. (SCI) (IF: 6.100; R/C=46/279 in PHARMACOLOGY & PHARMACY) Chang MC, Wang TM, Yeung SY, Jeng PY, Liao CH, Lin TY, Lin CC, Lin BR, Jeng JH. Antiplatelet effect by p-cresol, a uremic and environmental toxicant, is related to inhibition of reactive oxygen species, ERK/p38 signaling and thromboxane A2 production. Atherosclerosis. 2011, 219(2):559-65. (SCI) (IF: 6.847; R/C=11/67 in PERIPHERAL VASCULAR DISEASE) Chang-Hui Liao*, Jih-Jung Chen, Jieru Egeria Lin, Chia-Hsin Liu, Ching-Ping Tseng, Yuan-Ji Day. 5-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-3,7-dimethoxy-4H-chromen-4-one (MSF-2) suppresses fMLPmediated respiratory burst in human neutrophils by inhibiting phosphatidylinositol 3-kinase activity. Journal of cellular physiology, 2011, 226(6):1519-30. (SCI) (IF: 6.513; R/C=10/81 in PHYSIOLOGY) Liou BJ, Chang HS, Wang GJ, Chiang MY, Liao CH, Lin CH, Chen IS. Secondary metabolites from the leaves of Neolitsea hiiranensis and the anti-inflammatory activity of some of them. Phytochemistry. 2011, 72(4-5):415-22. (SCI) (IF: 4.004; R/C=161/296 in BIOCHEMISTRY & MOLECULAR BIOLOGY)