劉軒

劉軒

職稱：教授

研究室名稱：癌症功能基因體學實驗室

最高學歷：博士.

學校/國家：長庚大學/台灣

分機號碼：3526

電子郵件帳號：liu-hsuan@mail.cgu.edu.tw

個人網頁網址：https://cgmmrc.cgu.edu.tw/p/406-1029-69430,r1661.php?Lang=zh-tw

研究室現有：博士後研究員1人

博士班研究生1人

碩士班研究生3人

專任研究助理5人

大學部專題生3人

Update：2021/09/13

研究方向及研究室特色：

高通量(high-throughput)生物實驗技術揭開後基因體世代 (post-genomic era)的序幕，而近年呈現爆炸性發展的次世代定序生物技術，更進一步將生物實驗技術推向高效率、高精度、高準確性之境界，為生命科學研究領域創造極佳的契機。次世代定序方法目前也已被廣泛應用於各類型的生命科學研究工作上，研究人員可針對DNA與RNA進行高速定序，搭配後端生物資訊 (bioinformatics)的應用，進行基因序列變異分析、基因結構異常分析、基因表現量分析、微小RNA (small RNA)表現量分析、基因體甲基化修飾等之研究。

本實驗室的研究主要在建立和應用各種NGS技術平台於癌症基因體及轉錄體上的研究，搭配生物資訊的分析, 系統性的找出癌症重要的“生物特徵”及相關病理特徵，利用大數據庫運算，建構其可能參與之調控網絡，並利用細胞和動物模式進行深入的分子機制探討及驗証。近年主要研究包括：

  1. 建立台灣本土癌症組織之功能性基因體體學數據庫

建立完整的台灣本土癌症組織(口腔癌/食道癌/大腸直腸癌/腦癌)之功能性基因體體學數據庫，並利用生物資訊方式，結合國外大數據庫，系統性的分析找出與臨床的早期診斷、疾病的治療與用藥、抗藥性、存活率與再復性相關的重要分子，完整的描繪這些變體的病理作用機制以及調控網絡。針對其中重要的變異基因，進一步使用細胞和動物模式研究來深入探討及驗証這些癌症相關標的之分子機制。

  1. 開發分析非編碼RNA調控網絡之生物資訊工具

利用非編碼RNA已知之生物特性 (long noncoding RNA/circRNA/miRNA)，結合大數據庫，開發分析非編碼RNA調控網路之生物資訊分析平台。進一步結合細胞和動物模式，驗証其中新穎之非編碼RNA的調控分子機制。

  1. 開發次世代定序法於癌症液態檢體之應用

外泌體組成不僅反應了起源細胞，且富含與細胞轉化相關的生物分子，因此已成為近年來液體活檢方法中最有潛力的目標。實驗室建構了一套只需1 cc的血漿，即可快速提取外泌核糖核酸方法，利用次世代定序法驗証了此方法明顯優化並提升樣本製備的穩定性。我們更進一步全面性的建立了健康人及多種癌症之外泌微核糖核酸體學數據庫 (口腔癌、食道癌、肺癌、大腸直腸癌)，結合臨床資訊，系統性的尋找具早檢測、長期監測治療反應以及預後之多面向的生物標誌。因應精準醫學方面之應用，更進一步開發一套快速檢測晶片系統，可精準且多面向的監測癌症及治療進程。利用多種生物資訊工具，完整描繪出外泌微核糖核酸在癌症進程所扮演的功能角色，並在細胞及動物模式上進行深入的機制研究。

 

最近五年所發表論文：

  1. Chiang S-F, Huang HH, Tsai W-S, Chin-Ming Tan B, Yang C-Y, Huang P-J, Yi-Feng Chang I, Lin J, Lu P-S, Chin E, Liu Y-H, Yu J-S, Chiang J-M, Hung H-Y, Liu H* (2021, Mar). Comprehensive functional genomic analyses link APC somatic mutation and mRNA-miRNA networks to the clinical outcome of stage-III colorectal cancer patients. Biomedical Journal, Available online 16 March 2021. MOST 106-2320-B-182-035-MY3. 本人為通訊作者. (SCI)
  2. Min-Hua Tseng, Wen-Lang Fan, Hsuan Liu, Chia-Yu Yang, Jhao-Jhuang Ding , Hwei-Jen Lee , Shih-Ming Huang, Shih-Hua Lin and Jing-Long Huang (2021, Feb). Complement Factor I Mutation May Contribute to Development of Thrombotic Microangiopathy in Lupus Nephritis. Frontiers in Medicine, Feb 5;7:621609. (SCI)
  3. Wen-Sy Tsai, Wei-Shan Hung, Tzu-Min Wang, Hsuan Liu, Chia-Yu Yang, Shao-Min Wu, Hsueh-Ling Hsu, Yu-Chiao Hsiao, Hui-Ju Tsai, Ching-Ping Tseng (2020, Oct). Circulating tumor cell enumeration for improved screening and disease detection of patients with colorectal cancer. Biomedical Journal. MOST 106-2320-B-182-027-MY3. (SCI)
  4. Chen YT, Chang IY, Kan CH, Liu YH, Kuo YP, Tseng HH, Chen HC, Liu H, Chang YS, Yu JS, Chang KP, Tan BC. (2020, Aug). circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response. Cells, 10;9(8):1868. MOST 106-2320-B-182-035-MY3. (SCI)
  5. Yang CY, Liu CR, Chang IY, OuYang CN, Hsieh CH, Huang YL, Wang CI, Jan FW, Wang WL, Tsai TL, Liu H, Tseng CP, Chang YS, Wu CC, Chang KP (2020, Jun). Cotargeting CHK1 and PI3K Synergistically Suppresses Tumor Growth of Oral Cavity Squamous Cell Carcinoma in Patient-Derived Xenografts. Cancers (Basel), 29;12(7):1726.. MOST 107-2314-B-182-075-MY3. (SCI)
  6. Wu SM, Tsai WS, Chiang SF, Lai YH, Ma CP, Wang JH, Lin J, Lu PS5, Yang CY, Tan BC, Liu H* (2020, Mar). Comprehensive transcriptome profiling of Taiwanese colorectal cancer implicates an ethnic basis for pathogenesis. Sci Rep, 10(1):4526. MOST 106-2320-B-182-035-MY3.本人為通訊作者. (SCI)
  7. Chung AK, OuYang CN, Liu H, Chao M, Luo JD, Lee CY, Lu YJ, Chung IC, Chen LC, Wu SM, Tsang NM, Chang KP, Hsu CL, Li HP, Chang YS. (2019, Sep). Targeted sequencing of cancer-related genes in nasopharyngeal carcinoma identifies mutations in the TGF-β pathway. Cancer Medicine, 8(11):5116-5127. MOST 105-2320-B-182-034-MY3. (SCI)
  8. Kuo YP, Ma CP, Chen HW, Chen YT, Lai YH, Liu H, Kuo RL, Chin-Ming Tan B. (2019, Sep). A novel antisense RNA ASPACT confers multi-level suppression of PACT and associated signalling. RNA Biology, 16(9):1263-1274. (SCI).
  9. Ma CP, Liu H, Yi-Feng Chang, Wang WC, Chen YT, Wu SM, Chen HW, Kuo YP, Shih CT, Li CY, Tan BC. (2019, May). ADAR1 promotes robust hypoxia signaling via distinct regulation of multiple HIF-1α-inhibiting factors.EMBO Report, 20(5): pii: e47107. MOST 106-2320-B-182-042-MY3. (SCI)
  10. Lai CY*, Liu H*, Tin KX, Huang Y, Yeh KH, Peng HW, Chen HD, He JY, Chiang YJ, Liu CS, Weng SY, Tao MH, Yen JJ, Yang-Yen HF. (2019, Jan). Identification of UAP1L1 as a critical factor for protein O-GlcNAcylation and cell proliferation in human hepatoma cells. . Oncogene, 38(3):317-331.. MOST 102-2320-B-001-024-MY3. 本人為第一作者. (SCI)
  11. You GR, Cheng AJ, Lee LY, Huang YC, Liu H, Chen YJ, Chang JT. (2019, Jan). Prognostic signature associated with radioresistance in head and neck cancer via transcriptomic and bioinformatic analyses. BMC Cancer, 19(1):64. (SCI).
  12. Huang YW, Chiang MF, Ho CS, Hung PL, Hsu MH, Lee TH, Chu JL, Liu H, Tang P, Ng VW, Lin DS (2018, Dec). A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder. Aging and Disease, 9(6):1043-1057. (SCI).
  13. Chao A, Lin CY, Wu RC, Lee YS, Lee LY, Tsai CL, Yang LY, Liu H, Chen SJ, Wang TH, Lai CH (2018, Nov). The combination of everolimus and terameprocol exerts synergistic antiproliferative effects in endometrial cancer: molecular role of insulin-like growth factor binding protein 2. J Mol Med (Berl), 96(11):1251-1266. MOST 105-2320-B-182A-005. (SCI).
  14. Chang S, Bok P, Tsai CY, Sun CP, Liu H, Deussing JM, Huang GJ (2018, Aug). NPTX2 is a key component in the regulation of anxiety. Neuropsychopharmacology, 43(9):1943-1953. (SCI).
  15. Chen YT, Chang IY, Liu H, Ma CP, Kuo YP, Shih CT, Shih YH, Kang L, Tan BC (2018, Jun). Tumor-associated intronic editing of HNRPLL generates a novel splicing variant linked to cell proliferation. J Biol Chem, 293(26) 10158–10171. MOST 105-2320-B-182-017. (SCI).
  16. Yang CY, Yeh YM, Yu HY, Chin CY, Hsu CW, Liu H, Huang PJ, Hu SN, Liao CT, Chang KP, Chang YL. (2018, May). Oral Microbiota Community Dynamics Associated With Oral Squamous Cell Carcinoma Staging. Front Microbiol, 9:862. (SCI).
  17. Tsai KW, Lo YH, Liu H, Yeh CY, Chen YZ, Hsu CW, Chen WS, Wang JH (2018, Mar). Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer. Molecular Cancer, 17(1):72. (SCI).
  18. Lai YH, Liu H, Chiang WF, Chen TW, Chu LJ, Yu JS, Chen SJ, Chen HC, Tan BC (2018, Jan). MiR-31-5p-ACOX1 Axis Enhances Tumorigenic Fitness in Oral Squamous Cell Carcinoma Via the Promigratory Prostaglandin E2. Theranostics, 8(2):486-504. MOST 106-2320-B-182-035-MY3. (SCI).
  19. Wu SM*, Liu H*, Huang PJ, Chang IY, Lee CC, Yang CY, Tsai WS, Tan BC. (2018, Jan). circlncRNAnet: An integrated web-based resource for mapping functional networks of long or circular forms of non-coding RNAs. , 7 (1):1-10. MOST 106-2320-B-182-035-MY3. 本人為第一作者(SCI).
  20. Chen TW*, Lee CC*, Liu H*, Wu CS*, Pickering CR*, Huang PJ, Wang J, Chang YF, Yeh YM, ChenCD, Li HP, Luo JD, Tan BC, Chan EH, Hsueh C, Chu LJ, Chen YT, Zhang B, Yang CY, Wu CC, Hsu CW, See LC, Tang P, Yu JS, Liao WC, Chiang KW, Rodriguez H, Myers JN., Chang KP, Chang YS (2017, Sep). APOBEC3A is an oral cancer prognostic biomarker in Taiwanese carriers of an APOBEC deletion polymorphism. Nature Communication, 8 (1):465.. MOST 103-2632-B-182-001. 本人為第一作者. (SCI)
  21. Yang CC, Chen YT, Chang YF, Liu H, Kuo YP, Shih CT, Liao WC, Chen HW, Tsai WS, Tan, BC (2017, May). ADAR1 mediated 3’ UTR editing and expression control of anti-apoptosis genes fine-tunes cellular apoptosis response. Cell Death and Disease, 8(5):e2833. MOST 105-2320-B-182-017. (SCI).
  22. Chen H, Hsiao YC, Chiang SF, Wu CC, Lin YT, Liu H, Zhao H, Chen JS, Chang YS, Yu JS. (2016, Aug). Quantitative analysis of wild-type and V600E mutant BRAF proteins in colorectal carcinoma using immunoenrichment and targeted mass spectrometry. Analytica Chimica Acta, 933:144-55. (SCI).

 
         

 

 

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