2025/09/25 Cryo-EM Introduction and Application for JEM-2100Plus Transmission Electron Microscope
2025/09/25 (Thr)--Cryo-Electron Microscopy (Cryo-EM) Workshop on JEOL JEM-2100Plus Transmission Electron Microscope
(Cryo-EM Introduction and Application for JEM-2100Plus Transmission Electron Microscope)
Introduction:
Biological specimens such as proteins, extracellular vesicles (EVs), and liposomes are often vulnerable to structural damage under conventional transmission electron microscopy (TEM) due to staining, dehydration, and electron beam radiation. Cryo-electron microscopy (cryo-EM) overcomes these limitations by rapidly plunge-freezing the samples into a layer of vitreous ice, thereby preserving their native structures in a near-physiological state at cryogenic temperatures (approximately -180°C). The JEOL JEM-2100Plus TEM supports cryo-EM imaging and is equipped with the standard Digital Micrograph (DM) software for image acquisition. Additionally, the Shot Meister software enhances imaging of beam-sensitive specimens under low-dose conditions, achieving high-contrast and resolution images that facilitate downstream data processing of 3D reconstruction. In recent years, remote access and automation have become increasingly important in the field of electron microscopy, especially in response to the global pandemic. To meet this demand, JEOL Japan has developed PyJEM, a Python-based remote control platform for JEOL TEM/STEM instruments. Compared to traditional C++-based control systems, Python’s intuitive syntax simplifies the development of control scripts and lowers the learning barrier for non-programmers.
Key features of PyJEM include:
˙Optical system control: Beam settings, magnification, brightness, and switching between STEM/EDS detectors
˙Stage control: Absolute/relative movements and piezoelectric positioning
˙Automated adjustments: Autofocus, astigmatism correction, contrast and brightness optimization
˙Custom experimental workflows: Enables TEM operations not supported by DM Script
˙Offline simulation: PyJEM. offline module allows users to develop and test scripts on non-networked computers without the connection of TEM
We welcome all faculty members and students to attend this Cryo-EM training course. If you have any questions, please contact Ms. He at extension #5065.
Cryo-EM Principles and Software Introduction (Oral Presentation):
Date: Thursday, September 25, 2025
Time: 10:30 - 12:00
Venue: Conference Hall I, B1, Second Medical Building
Speaker: Mr. Yieng-Chieh Hung, Manager, Application Engineering Department, Jiedong Ltd.
Demonstration of Room Temperature TEM Operation:
Date: Thursday, September 25, 2025
Time: 13:10-14:10
Venue: Room 0459, 4th Floor, Zone B, Microscopy Center, First Medical Building
Demonstration of Cryo-EM Sample Preparation by Cryo-Plunge 3:
Date: Thursday, September 25, 2025
Time: 3:10 PM – 5:10 PM (or 10:00 AM – 12:00 PM the following day)
Venue: Room 0460, 4th Floor, Zone B, Microscopy Center, First Medical Building
※Note: Due to the need for pre-cooling the CP3 system, this session will be held based on the number of registered participants.
An additional session may be arranged and announced via email. Limited space available—early registration is strongly recommended.
Laboratories are encouraged to send representatives.
Online Registration Link:
https://forms.gle/Hhy61xGCByenxpDH8
※Kindly respond before 09/23 (Tue). Thank you for your cooperation. Contact: Wan-Zhen He, Phone: #5065