Instrument introduction:
1. Brand type:
One Germany LEICA STELLARIS8.
One Germany ZEISS LSM780.
One Germany ZEISS LSM510 META NLO.
2. Main functions:
Germany LEICA STELLARIS8
1. Diode Laser: 405 nm 50mW
2. White light laser system, pulsed laser 440nm-790nm, 1nm adjustable, 78MHz.
3. TauSense (TauContrast, TauGating, TauSeperation)
4. Power HyDS (3 set), Power HyDX (1 set)
5.Falcon FLIM application
6. Lightning confocal superresolution
7. LAS X Navigator application
Germany ZEISS LSM780
1. Diode Laser: 405 nm 30mW
2. CW Laser: 440 nm 15mW
3. Multiline Argon-Laser: 458/488/514 nm 25mW
4. DPSS Laser: 561 nm 20mW
5. HeNe Laser: 633 nm 5mW
Germany ZEISS LSM510 META NLO
1. Mai-Tai HP laser (Spectra-Physics): 690-1020 nm
2. Multiline Argon-Laser: 458/477/488/514 nm 30mW
3. DPSS Laser: 561 nm 10mW
4. HeNe Laser: 633 nm 5mW
Placement:
"Microscope Center", Room 0459, Area B, 4th Floor, First Medical Building.
Opening hours:
Monday to Friday AM 8:30～PM 5:00
Application principles:
A. Equipment Booking:
1. Please visit the Microscope Center website to register and book your usage time, and operate the equipment as scheduled.
2. If admin assistance is required, confirm the operating time with the admin after booking on the website. Charges will be based on the booking time recorded on the website.
B. Those who board the plane by themselves:
1. Please go to the microscope center to register and make an appointment, and get on the machine at the agreed time.
2. Technical appraisal procedure: Make an appointment with the contact person of the center for practice time, and further accept the operation appraisal.
3. Technical appraisal standards:
a. Understanding of the working principle of the machine.
b. Preparations before starting up. (Operation of microscope, inquiry of work records)
c. Test items after startup. (Laser, machine condition test)
d. The relationship between software and machines.
e. Accurately record usage status.
f. Troubleshooting.
C. If admin assistance is required during operation, please make a note in the remarks column.
For full-time admin support, schedule the session for Thursday mornings (9:00 AM–12:00 PM), note it in the remarks column, and notify the admin. (e.g., LEICA STELLARIS8 user)
D. Notes:
1. If you do not get on the plane for 1 hour after the scheduled time, the center may arrange this time for other people who need to get on the plane. If you find that there is a problem with the experiment and you are unable to board the machine, please cancel the reservation, otherwise the reservation period will be charged according to the price.
2. Anyone who violates the usage regulations three times will be disqualified and will not be allowed to apply again within six months.
3. The experimental results provided by our center are for academic research reference only and we do not assume any legal responsibility.
4. The above usage and charging methods will be revised according to the situation. The actual situation will be subject to the online announcement.
5. Turn on and off the machine: Please follow the correct procedure.
6. Data backup:
(1) Users should save the experimental data immediately after each experiment.
(2) The administrator removes user data from the computer every six months.
E paper publication and statement:
1. The services of this Microscopy Center are mainly academic research. Anyone who applies to this laboratory for the listed services must be stated in the acknowledgment column of the article when the results are published.
2. If you cooperate with teachers of our school and sign a cooperation contract, the method of sharing the results will be in accordance with the content of the contract.
Charging principles:
1.Charge standard:
LEICA STELLARIS8
Fee items unit price (on campus) / unit price (off campus, no cooperation plan)
Instrument usage fee: 850 NT/hour / 1700 NT hour
The charging principle for long-term observation of living cells is that between PM 08:00 on the same day and AM 08:00 on the next day, if the reservation is continued for more than 5 hours, the fee is 500 yuan per hour.
If operator assistance from an admin is required, please note it in the remarks column.
For full-time admin support, schedule it for Thursday mornings (9:00 AM–12:00 PM), add it to the remarks column, and inform the admin. (e.g., LEICA STELLARIS8 user)
ZEISS LSM780
Fee items unit price (on campus) / unit price (off campus, no cooperation plan)
Instrument usage fee: 650 NT/hour / 1300 NT hour
ZEISS LSM510 META NLO
Fee items unit price (on campus) / unit price (off campus, no cooperation plan)
Instrument usage fee: 500 NT/hour / 1000 NT hour
Note: Charges are calculated based on the reservation time, and the part less than half an hour is calculated as half an hour. If the delay is caused by the former use, it will not be included.
2.Charging method:
Transfer payment: For research projects within the Chang Geng system, the research project leader should confirm, sign and stamp the fee schedule for the use of each instrument immediately upon receiving it, and then submit it to the accounting office of the center according to the plan. Case number transfer payment.
Cash payment: For research projects that are not under the Chang Gung Memorial System, payment will be made to the cashier of the school according to the payment slip issued by each instrument contact person. If a receipt is required, the payment will be issued at the accounting office.
Contact person:
Mr. Peng Ji-lung
Tel: (03)211-8800 ext. 5065; E-mail: pon@mail.cgu.edu.tw