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流式細胞分選儀(FACSAriaIIu)

服務簡介1
BECTON DICKINSON  FACSAriaIIu
(雷射波長375 or 405 & 488 & 635nm)


ANALYSIS:
including cell surface and cytoplasma analysis

    (1)Cell Marker Analysis 

    (2)Cell Cycle Analysis 

    (3)Apoptosis and Necrosis

    (4)Free Radical (ROS)

    (5)Membrane Potential

        

SORTING:

    (1)一般 Sorting

    (2)無菌 Sorting

    (3)ACDU (clony:cell/well)


呂雅芬 小姐#

分機:
5428


FACSAriaIIu_4-laser, 9 colour

Excitation Laser

Dectection Filter

example

 488nm Blue Laser

530/30

Alexa Fluor 488, FITC, BB515

575/26

PE

610/20

PE-Texas Red

695/40

PI, PerCP, PerCP-Cy5.5, BB700

780/60

PE-Cy7

633nm Red Laser

660/20

APC, Alexa Fluor 647

780/60

APC-Cy7, APC-H7

375nm Near UV Laser
or
405nm Violet Laser

450/40

DAPI / Hoechst Blue (Cell cycle)

670LP; 510/50(502LP)

Hoechst Red (Side population)


流式細胞分析儀(FACSCalibur)

FACSCalibur
BECTON DICKINSON  FACSCalibur 一台
(雷射波長488 & 635nm)


ANALYSIS
including cell surface and cytoplasma analysis

    (1)Cell Marker Analysis 

    (2)Cell Cycle Analysis 

    (3)Apoptosis and Necrosis

    (4)Free Radical (ROS)

    (5)Membrane Potential



呂雅芬 小姐#

分機:
5428


FACSCalibur_2-laser, 4 colour

Excitation Laser

Dectection Filter

example

 488nm Blue Laser

530/30

Alexa Fluor 488, FITC, BB515

575/26

PE,PI

> 670

PerCP, PerCP-Cy5.5, BB700

633nm Red Laser

661/16

APC, Alexa Fluor 647


聲波聚焦流式細胞儀(Attune NxT)

Attune NxT
ThermoFisher  Attune NxT一台
(雷射波長405 & 488 & 561 & 635nm)


ANALYSIS
including cell surface and cytoplasma analysis

    (1)Cell Marker Analysis 

    (2)Cell Cycle Analysis 

    (3)Apoptosis and Necrosis

    (4)Free Radical (ROS)

    (5)Membrane Potential

    (6)whole blood analysis
       (No-Lyse-No-Wash)



呂雅芬 小姐#

分機:
5428


Attune NxT_4-laser, 14 colour

Excitation Laser

Dectection Filter

Channel

example

405nm Violet Laser

440/50

VL1

Alexa Fluor 405, Pacific Blue

512/25

VL2

Pacific Green

603/48

VL3

Pacific Orange, Qdot*605

710/50

VL4

Qdot*705

 488nm Blue Laser

530/30

BL1

Alexa Fluor 488, FITC

575/26

BL2

PE-Alexa Fluor 610, PE, PE-Texas Red

695/40

BL3

PerCP, PE-Cy5.5, PerCP-Cy5.5

561nm Yellow Laser

585/16

YL1

PE

620/15

YL2

PE-Texas Red, PE-Alexa Fluor 610

695/40

YL3

PE-Cy5.5,PE-Alexa Fluor 700

780/60

YL4

PE-Cy7,Qdot*800

637nm Red Laser

670/14

RL1

APC, Alexa Fluor 647

720/30

RL2

Alexa Fluor 680, APC-Alexa Fluor 700

780/60

RL3

APC-Cy7,APC-Alexa Fluor 750


流式細胞多色分析儀(BD LSR II)

LSR II
BECTON DICKINSON BD LSR II一台
(雷射波長405 & 488 & 635nm)


ANALYSIS
including cell surface and cytoplasma analysis

    (1)Cell Marker Analysis 

    (2)Cell Cycle Analysis 

    (3)Apoptosis and Necrosis

    (4)Free Radical (ROS)

    (5)Membrane Potential




呂雅芬 小姐#

分機:
5428


BD LSR II_3-laser, 11 colour

Excitation Laser

Dectection Filter

example

 488nm Blue Laser

530/30

Alexa Fluor 488, FITC, BB515

575/26

PE

610/20

PE-Texas Red

660/20

PE-Cy5

695/40

PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD

780/60

PE-Cy7

633nm Red Laser

670/30

APC, Alexa Fluor 647

730/45

Alexa Fluor 700

780/60

APC-Cy7, APC-H7

405nm Violet Laser

450/50

Alexa Fluor 405, V450, BV421, PB

525/50

Alexa Fluor 430, V500, BV510


自動化細胞影像分析儀(LionHeart FX)

LionHeart FX
Agilent BioTek LionHeart FX 一台
(LED cube DAPI & GFP & TexasRED & CY5)

可拍攝細胞或組織切片的螢光、一般明視野、高對比明視野、彩色明視野與長時間觀測等影像,進行縫圖、疊圖、去背景或影片生成等處理,最後獲得定性或定量資料。


謝翠芝 小姐#

分機:
5191


LionHeart FX

1.Lamp

High power LED

2.Objective

Zeiss 2.5X/0.12

Olympus 4X/0.1310X/0.320X/0.4540X/0.6

3.Plate type

Observable 6-384 plateslidechamber slide

3.5cm dish6cm dish

4.Environment

Room temperature to 40and CO2 5%

5.Filter cube

Excitation

Emission

DAPI

377/50X

447/60M

GFP

469/35X

525/39M

TexasRED

586/15X

647/57M



高通量生物分子交互作用分析儀 (Octet RED96)

Octet RED96

SARTORIUS Octet RED96一台


利用BLI(生物膜光干涉技術)觀察生物分子間的交互作用,進行生物分子的定量與生物分子間動力學、親和力的分析(kakdKD)。檢測結束後,樣品可以回收利用。

可用於抗原 - 抗體;蛋白質分子之間以及藥物-蛋白質;受體 - 配體;核酸與蛋白質之間;核酸-核酸等分子之間交互作用的分析





謝翠芝 小姐#

分機:
5191


Octet RED96

1.Affinity

Range1mM-10pM

2.Molecular Weight

150Da

3.Minimum Sample Volume

200uL

4.Maxmum Simultaneous Reads

8

5.Temperature Control

Ambient+4℃〜40


高通量多功能盤式分析儀 (EnSpire 2300)

EnSpire 2300

PerkinElmer EnSpire 2300一台


可測定類別為ALPHA 、冷光及可見光。





謝翠芝 小姐#

分機:
5191


EnSpire ALPHA 2300

1.ALPHA

Excitation 680nm

Emission 615nm

2.Luminescence

3.Absorption

Visible light 260nm

Visible light 280nm

Visible light 405nm

Visible light 450nm

Visible light 540nm

Visible light 595nm



高通量細胞影像分析系統(INCell Analyzer 1000)


INCell 1000
GE INCell Analyzer 1000一台
GE Investigator 一套

IN Cell Analyzer 1000

    (1)Lamp:Xenon 100W 
    (2)Microscope:Nikon TE 2000 
    (3)Objective:4X/0.2、10X/0.45、
                             20X/0.45、40X/0.6
    (4)CCD Camera
    (5)Plate type:6-96 well plate
Investigator
    (1)Workstation version 3.6
    (2)Developer version 1.8


謝翠芝 小姐#

分機:
5191



INCell Analyzer 1000

1.Lamp

Xenon 100W

2.Objective

Nikon 4X/0.210X/0.4520X/0.4540X/0.6

3.Plate type

Observable 6-96 plateslide

4.Filter

Excitation

Emission

350/50X

455/50M

480/40X

525/36M

555/25X

620/60M

645/30X

705/72M



X光片洗片機(X-ray film processor)

X光洗片機

Protec Optimax, Film Processor二台

可沖洗之X光片尺寸最大為 35x43 cm(14x17 inch)最小為10x10 cm (4x4 inch) 洗片時程約30~60秒



廖修遠 先生#

分機:
5970

消毒鍋(Sterilizer)


GETINGE 733HC 955   CASTLE MC 3533 110
GETINGE 733HC 955公升兩台

CASTLE M/C 3533 250公升一台

可設定溫度 (100~135℃) 及乾燥時間 (overnight)。平均每次消毒作業約需 60~90 分鐘(乾燥時間另計)




温宜霖 先生#

分機:
5256