流式細胞儀設備
流式細胞分選儀 BECTON DICKINSON FACSAriaIIu
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FACSAriaIIu_4-laser, 9 colour |
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Excitation Laser |
Dectection Filter |
example |
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488nm Blue Laser |
530/30 |
Alexa Fluor 488, FITC, BB515 |
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575/26 |
PE |
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610/20 |
PE-Texas Red |
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695/40 |
PI, PerCP, PerCP-Cy5.5, BB700 |
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780/60 |
PE-Cy7 |
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633nm Red Laser |
660/20 |
APC, Alexa Fluor 647 |
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780/60 |
APC-Cy7, APC-H7 |
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375nm Near UV Laser |
450/40 |
DAPI / Hoechst Blue (Cell cycle) |
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670LP; 510/50(502LP) |
Hoechst Red (Side population) |
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流式細胞分析儀 BECTON DICKINSON FACSCalibur
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FACSCalibur_2-laser, 4 colour |
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Excitation Laser |
Dectection Filter |
example |
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488nm Blue Laser |
530/30 |
Alexa Fluor 488, FITC, BB515 |
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575/26 |
PE,PI |
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> 670 |
PerCP, PerCP-Cy5.5, BB700 |
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633nm Red Laser |
661/16 |
APC, Alexa Fluor 647 |
流式細胞多色分析儀 ThermoFisher Attune NxT
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Attune NxT_4-laser, 14 colour |
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Excitation Laser |
Dectection Filter |
Channel |
example |
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405nm Violet Laser |
440/50 |
VL1 |
Alexa Fluor 405, Pacific Blue |
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512/25 |
VL2 |
Pacific Green |
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603/48 |
VL3 |
Pacific Orange, Qdot*605 |
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710/50 |
VL4 |
Qdot*705 |
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488nm Blue Laser |
530/30 |
BL1 |
Alexa Fluor 488, FITC |
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575/26 |
BL2 |
PE-Alexa Fluor 610, PE, PE-Texas Red |
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695/40 |
BL3 |
PerCP, PE-Cy5.5, PerCP-Cy5.5 |
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561nm Yellow Laser |
585/16 |
YL1 |
PE |
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620/15 |
YL2 |
PE-Texas Red, PE-Alexa Fluor 610 |
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695/40 |
YL3 |
PE-Cy5.5,PE-Alexa Fluor 700 |
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780/60 |
YL4 |
PE-Cy7,Qdot*800 |
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637nm Red Laser |
670/14 |
RL1 |
APC, Alexa Fluor 647 |
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720/30 |
RL2 |
Alexa Fluor 680, APC-Alexa Fluor 700 |
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780/60 |
RL3 |
APC-Cy7,APC-Alexa Fluor 750 |
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流式細胞多色分析儀 BD LSR II
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BD LSR II_3-laser, 11 colour |
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Excitation Laser |
Dectection Filter |
example |
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488nm Blue Laser |
530/30 |
Alexa Fluor 488, FITC, BB515 |
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575/26 |
PE |
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610/20 |
PE-Texas Red |
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660/20 |
PE-Cy5 |
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695/40 |
PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD |
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780/60 |
PE-Cy7 |
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633nm Red Laser |
670/30 |
APC, Alexa Fluor 647 |
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730/45 |
Alexa Fluor 700 |
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780/60 |
APC-Cy7, APC-H7 |
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405nm Violet Laser |
450/50 |
Alexa Fluor 405, V450, BV421, PB |
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525/50 |
Alexa Fluor 430, V500, BV510 |
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