Instrumentation Center

The automated imaging system is designed to capture various types of images—including fluorescence, brightfield, high-contrast brightfield, and color brightfield—of cells and tissue sections. It enables advanced image processing such as stitching, stacking, and background subtraction, ultimately generating both high-quality images and corresponding analytical data.   Title: Automated Cell Imaging Optical Analysis System (LionHeart FX) Training Courses Date: May 06, 2025 (Tuesday) 10:10 AM - 11:30 AM Location: M201 Classroom, 2nd Floor, First Medical Building Speaker: Ms. Yu-Hui Jiang, Field Application Scientist (Agilent Technologies Taiwan) Live Demonstration: High Throughput Analysis Room, 9th Floor, First Medical Building (R:0906) Demonstration Time: Same afternoon, conducted in two sessions, each lasting 1 hour Registration Link:https://forms.office.com/r/brhQD9dsZH(Deadline: May 02, 2025) Contact Email:iris@mail.cgu.edu.tw   All are welcome to sign up and participate!

For improving our center research effectiveness evaluation basis, we are going to remind PI at center  homepage and quarterly charge-fee record sheet to mention the usage of our center’s equipment and resources in "Acknowledgment" when your papers are published. The full center name as follow： Instrumentation Center, Chang Gung University, Tao-Yuan, Taiwan.

Automated Cell Imaging Optical Analysis System (LionHeart FX) Training The automated imaging system can capture images of cells and tissue sections in fluorescence, bright field, high-contrast bright field, and color bright field. It performs image processing such as stitching, overlaying, or background removal, ultimately analyzing the images to obtain qualitative or quantitative data. Title: Automated Cell Imaging Optical Analysis System (LionHeart FX) Training Date: November 20, 2024 (Wednesday) 10:10 AM - 11:30 AM Location: M306 Classroom, 3rd Floor, First Medical Building Speaker: Ms. Yu-Hui Jiang, Field Application Scientist (Agilent Technologies Taiwan) Live Demonstration: High Throughput Analysis Room, 9th Floor, First Medical Building (R:0906) Demonstration Time: Same afternoon, conducted in two sessions, each lasting 1 hour Registration Link: https://forms.office.com/r/ERNwyi6u5s (Deadline: November 15, 2024) Contact Email: iris@mail.cgu.edu.tw We welcome you to register and participate!

The reservation system of the technical service platform has been established and online reservations will be available from now on. System URL：https://insreservation.cgu.edu.tw/ Contact：Ms. Tsui-Chih Hsieh Tel：03-2118800#5191

If you have used the instruments in High-Throughput Analysis Laboratory and published papers, please send the paper's PDF file to our laboratory administrator.(iris@mail.cgu.edu.tw) To facilitate the collection of statistics. Infinitely grateful!

Automated camera system for cell fluorescence, bright field, high contrast bright field and color bright field. Perform image processing such as stitching, overlaying, or background removal. Final analysis to obtain qualitative or quantitative data. Specifications are as follows： 1.Light source：High power LED。 2.Focus mode: Image autofocus 3.Camera resolution: 16bit grayscale Sony CMOS 4.Objective：2.5X/0.12, 4X/0.13, 10X/0.3, 20X/0.45, 40X/0.6。 5.Filter：DAPI 377/50X, GFP 445/45X, Texas Red 586/15X, CY5 628/40X 6.Environment control：Room temperature to 400C. 7.Automatic displacement platform：Observable 6-384 plate、slide、chamber slide、3.5 cm dish、6 cm dish。 8.Image processing and analysis report using Gen5 software   Principle of use： 1.Open the Chang Gung system to make appointments during business hours. 2.Participate in the relevant education and training. After pass the operation assessment, then you can make an appointment to use it by yourself. 3.Instrument usage fee： Fee list CGU Labs Global Research & Industry Alliance Non CGU Labs Use by userself  50 NTD/hour -- -- Entrust to use 200 NTD/hour 750 NTD/hour 1000 NTD/hour 4.After using it, please fill in the usage record form. 5.For detailed usage rules, refer to [LionHeartFX Usage Points]. 6.Contact: Ms. Tsui-chih Hsieh      Tel:(03) 211-8800 ext. 5191      Email:iris@mail.cgu.edu.tw.

The interaction between biomolecules and biomolecules was analyzed by BLI (BioLayer Interferometry)  technique. Quantification of biomolecules, Biomolecular dynamics and Analysis of affinity(ka, kd and KD)  were analyzed. After the test, the sample can be recycled. Application： Drug Discovery, Antibody Screening, Ligand Fishing, Binding Specificity, Protein Quantization,  Molecular Recognition, Cell Signal Transduction, Proteomics, Vaccine Development. Use principle： 1.Make a appointment on https://insreservation.cgu.edu.tw/en. 2.Open Chang Geng system colleagues in the working hours. 3.After the use, please fill out the use of the record form. 4.No instrument use fee, but please cherish to use. 5.Contact: Ms. Tsui-chih Hsieh      Tel:(03) 211-8800 ext. 5191      Email:iris@mail.cgu.edu.tw.

EnSpire Alpha 2300 can detect Alpha technology releasing fluorescent signal. According to the different research needs, you can elastic design your experiment.  Applications： Intermolecular interaction, Target drug screening, Cellular Immunotherapy, Enzyme activity analysis, Cell proliferation and Poison detection, Reporter gene detection. Use principle： 1.Can detect ALPHA Fluorescence 、Luminescence and Absorbent light.    （Visible light 260nm, 280nm, 405nm, 450nm, 540nm, 595nm） 2.Open to Chang Geng system colleagues usage in the working hours.  3.Please fill in the reservation form in Room 0906, 9th Floor, First Medical Building. 4.After the use, please fill out the use of the record form. 5.Contact: Ms. Tsui-chih Hsieh      Tel:(03) 211-8800 ext. 5191      Email:iris@mail.cgu.edu.tw.

Use the objective lens to take a large number of images with the automatic platform（IN Cell Analyzer 1000）, then identify fluorescent signal in the image to get Quantity, Fluorescence intensity, Area,Length and other numerical analysis results.（IN Cell Investigator） Applications: Cell Cycle Analysis, Neurite Outgrowth Analysis,  Object Intensity Analysis, Micronucleus Formation Analysis, Morphology Analysis, Nuclear Trafficking Analysis, Dual Area Object Analysis, Plasma Membrane Trafficking Analysis, Granularity Analysis, Multi Target Analysis. Use principle： 1.Discuss conditions with operator and fill out the appointment schedule and application form。 2.Open to Chang Geng system colleagues usage in the working hours.  3.The principle of charging: Fee list CGU Labs Global Research & Industry Alliance Non CGU Labs IN Cell Analyzer 1000 Entrust to use      100 NTD/hour Entrust to use     375 NTD/hour Entrust to use     500 NTD/hour Investigator Entrust to use      100 NTD/hour Entrust to use     375 NTD/hour Entrust to use     500 NTD/hour 4.Contact: Ms. Tsui-chih Hsieh      Tel:(03) 211-8800 ext. 5191      Email:iris@mail.cgu.edu.tw.