Service Item
BECTON DICKINSON FACSAriaIIu
|
BECTON DICKINSON FACSAriaIIu (Laser 375 or 405 & 488 & 635nm) |
ANALYSIS： (1)Cell Marker Analysis (2)Cell Cycle Analysis (3)Apoptosis and Necrosis (4)Free Radical (ROS) (5)Membrane Potential
SORTING： (1)General Sorting (2)Sterile Sorting (3)ACDU (clony：cell/well)
|
Ms. Ya-Fen Lu
|
|
FACSAriaIIu_4-laser, 9 colour |
||
|
Excitation Laser |
Dectection Filter |
example |
|
488nm Blue Laser |
530/30 |
Alexa Fluor 488, FITC, BB515 |
|
575/26 |
PE |
|
|
610/20 |
PE-Texas Red |
|
|
695/40 |
PI, PerCP, PerCP-Cy5.5, BB700 |
|
|
780/60 |
PE-Cy7 |
|
|
633nm Red Laser |
660/20 |
APC, Alexa Fluor 647 |
|
780/60 |
APC-Cy7, APC-H7 |
|
|
375nm Near UV Laser |
450/40 |
DAPI / Hoechst Blue (Cell cycle) |
|
670LP; 510/50(502LP) |
Hoechst Red (Side population) |
|
BECTON DICKINSON FACSCalibur
|
BECTON DICKINSON FACSCalibur 1 set (Laser 488 & 635nm) |
ANALYSIS： (1)Cell Marker Analysis (2)Cell Cycle Analysis (3)Apoptosis and Necrosis (4)Free Radical (ROS) (5)Membrane Potential
|
Ms. Ya-Fen Lu
|
|
FACSCalibur_2-laser, 4 colour |
||
|
Excitation Laser |
Dectection Filter |
example |
|
488nm Blue Laser |
530/30 |
Alexa Fluor 488, FITC, BB515 |
|
575/26 |
PE,PI |
|
|
> 670 |
PerCP, PerCP-Cy5.5, BB700 |
|
|
633nm Red Laser |
661/16 |
APC, Alexa Fluor 647 |
ThermoFisher Attune NxT
|
ThermoFisher Attune NxT 1 set (Laser 405 & 488 & 561 & 635nm) |
ANALYSIS： (1)Cell Marker Analysis (2)Cell Cycle Analysis (3)Apoptosis and Necrosis (4)Free Radical (ROS) (5)Membrane Potential
(6)whole blood analysis
|
Ms. Ya-Fen Lu
|
|
Attune NxT_4-laser, 14 colour |
|||
|
Excitation Laser |
Dectection Filter |
Channel |
example |
|
405nm Violet Laser |
440/50 |
VL1 |
Alexa Fluor 405, Pacific Blue |
|
512/25 |
VL2 |
Pacific Green |
|
|
603/48 |
VL3 |
Pacific Orange, Qdot*605 |
|
|
710/50 |
VL4 |
Qdot*705 |
|
|
488nm Blue Laser |
530/30 |
BL1 |
Alexa Fluor 488, FITC |
|
575/26 |
BL2 |
PE-Alexa Fluor 610, PE, PE-Texas Red |
|
|
695/40 |
BL3 |
PerCP, PE-Cy5.5, PerCP-Cy5.5 |
|
|
561nm Yellow Laser |
585/16 |
YL1 |
PE |
|
620/15 |
YL2 |
PE-Texas Red, PE-Alexa Fluor 610 |
|
|
695/40 |
YL3 |
PE-Cy5.5,PE-Alexa Fluor 700 |
|
|
780/60 |
YL4 |
PE-Cy7,Qdot*800 |
|
|
637nm Red Laser |
670/14 |
RL1 |
APC, Alexa Fluor 647 |
|
720/30 |
RL2 |
Alexa Fluor 680, APC-Alexa Fluor 700 |
|
|
780/60 |
RL3 |
APC-Cy7,APC-Alexa Fluor 750 |
|
BECTON DICKINSON BD LSR II
|
BECTON DICKINSON BD LSR II 1 set (Laser 405 & 488 & 635nm) |
ANALYSIS： (1)Cell Marker Analysis (2)Cell Cycle Analysis (3)Apoptosis and Necrosis (4)Free Radical (ROS) (5)Membrane Potential
|
Ms. Ya-Fen Lu
|
|
BD LSR II_3-laser, 11 colour |
||
|
Excitation Laser |
Dectection Filter |
example |
|
488nm Blue Laser |
530/30 |
Alexa Fluor 488, FITC, BB515 |
|
575/26 |
PE |
|
|
610/20 |
PE-Texas Red |
|
|
660/20 |
PE-Cy5 |
|
|
695/40 |
PI, PerCP, PerCP-Cy5.5, BB700, 7-AAD |
|
|
780/60 |
PE-Cy7 |
|
|
633nm Red Laser |
670/30 |
APC, Alexa Fluor 647 |
|
730/45 |
Alexa Fluor 700 |
|
|
780/60 |
APC-Cy7, APC-H7 |
|
|
405nm Violet Laser |
450/50 |
Alexa Fluor 405, V450, BV421, PB |
|
525/50 |
Alexa Fluor 430, V500, BV510 |
|
Agilent BioTek LionHeart FX
|
Agilent BioTek LionHeart FX 1 set (LED cube DAPI & GFP & TexasRED & CY5) |
Automated camera system for cell fluorescence, bright field, high contrast bright field and color bright field. Performimage processing such as sitching, overlaying or background removal. Final analysis to get qualitative or quantitative data. |
Ms. Tsui-Chih Hsieh#
Tel:
|
|
LionHeart FX |
||
|
1.Lamp |
High power LED |
|
|
2.Objective |
Zeiss 2.5X/0.12 |
|
|
Olympus 4X/0.13，10X/0.3，20X/0.45，40X/0.6 |
||
|
3.Plate type |
Observable 6-384 plate，slide，chamber slide， |
|
|
3.5cm dish，6cm dish |
||
|
4.Environment |
Room temperature to 40℃and CO2 5% |
|
|
5.Filter cube |
Excitation |
Emission |
|
DAPI |
377/50X |
447/60M |
|
GFP |
469/35X |
525/39M |
|
TexasRED |
586/15X |
647/57M |
|
CY5 |
628/40X |
685/40M |
SARTORIUS Octet RED96
|
SARTORIUS Octet RED96 1 set |
The interaction between biomolecules and biomolecular kinetic and affinity was analyzer by BLI（Bio-Layer Interferomerty）. The ka, kd and KD were analyzed. After the test, the sample can be recycled. Can be used for antigen-antibody, proteinmolecules and drug-protein, receptor-ligand, nuclear acid-nuclear acid and other molecules between the interaction analysis. |
Ms. Tsui-Chih Hsieh#
Tel:
|
|
Octet RED96 |
|
|
1.Affinity |
Range：1mM-10pM |
|
2.Molecular Weight |
＞150Da |
|
3.Minimum Sample Volume |
200uL |
|
4.Maxmum Simultaneous Reads |
8 |
|
5.Temperature Control |
Ambient+4℃〜40℃ |
PerkinElmer EnSpire 2300
|
PerkinElmer EnSpire 2300 1 set |
Measureable category is ALPHA 、Luminscence and Absorbance。
|
Ms. Tsui-Chih Hsieh#
Tel:
|
|
EnSpire ALPHA 2300 |
||
|
1.ALPHA |
Excitation 680nm |
Emission 615nm |
|
2.Luminescence |
||
|
3.Absorption |
Visible light 260nm |
Visible light 280nm |
|
Visible light 405nm |
Visible light 450nm |
|
|
Visible light 540nm |
Visible light 595nm |
|
High Content Analysis
|
GE INCell Analyzer 1000 1 set GE Investigator 1 set |
IN Cell Analyzer 1000
(1)Lamp：Xenon 100W |
Ms. Tsui-Chih Hsieh#
Tel: |
|
INCell Analyzer 1000 |
||
|
1.Lamp |
Xenon 100W |
|
|
2.Objective |
Nikon 4X/0.2，10X/0.45，20X/0.45，40X/0.6 |
|
|
3.Plate type |
Observable 6-96 plate，slide |
|
|
4.Filter |
Excitation |
Emission |
|
350/50X |
455/50M |
|
|
480/40X |
525/36M |
|
|
555/25X |
620/60M |
|
|
645/30X |
705/72M |
|
X-ray film processor
|
|
The rinseable X-ray sheet has a maximum size of 35x43 cm (14x17 inch) and a minimum of 10x10 cm (4x4 inch) for about 30 to 60 seconds. |
Mr. Hsiu-Yuan Liao
#Tel:5970
|
Sterilizer
|
CASTLE M/C 3533 250 liter 1 set |
Can set the temperature (100 ~ 135℃) and drying time (0 ~ 12 hours). The
average disinfection of each operation takes about 60 to 90 minutes (dry time
separately) |
Mr. Yi-Lin Wen#
Tel:
|