新興病毒分子醫學國際碩士學位學程

研究方向及研究室特色(Lab & Research Interest)

深入了解RNA病毒與宿主間的交互作用，為病毒性疾病的治療和防控提供理論和技術支持。

一、RNA代謝標記實驗：

分析感染後宿主核糖核酸蛋白複合的組成，找出參與病毒轉譯或複製的宿主蛋白；同時進行轉錄組分析，瞭解病毒感染對宿主基因表現的影響。

二、RNA病毒分子模型：

建立不同階段的病毒複製分子模擬系統，驗證實驗結果，並尋找抗病毒藥物的潛在靶點。

三、三代定序於病毒基因體的分析：

利用長片段定序，進行病毒基因體的直接定序，並預測RNA病毒基因體的二級結構。探討這些二級結構對病毒複製的影響，並開發針對病毒二級結構的藥物。

論文與著作(Publication):

https://scholar.google.com.tw/citations?user=oBcQRd8AAAAJ&hl=zh-TW

1.          Guan-Hong Wu, Kuo-Ming Lee, Chia-Yu Kao, Shin-Ru Shih (2023, Jun). The internal ribosome entry site determines the neurotropic potential of enterovirus A71. Microbes and Infection, 25(5):105107.

2.          Pei-Lan Shao, Hsiao-Chen Tu, Yu-Nong Gong, Hung-Yu Shu, Ralph Kirby, Li-Yun Hsu, Hui-Yee Yeo, Han-Yueh Kuo, Yi-Chia Huang, Yung-Feng Lin, Hui-Ying Weng, Yueh-Lin Wu, Chien-Chih Chen, Tzen-Wen Chen, Kuo-Ming Lee, Chung-Guei Huang, Shin-Ru Shih, Wei J Chen, Chen-Chi Wu, Chong-Jen Yu, Shih-Feng Tsai (2023, Apr). Emergence and Persistent Dominance of SARS-CoV-2 Omicron BA.2.3.7 Variant, Taiwan. Emerging Infectious Diseases, 29(4):792-796.

3.          Kuo-Ming Lee, Syh-Jae Lin, Chung-Jung Wu, Rei-Lin Kuo (2023, Feb). Race with virus evolution: The development and application of mRNA vaccines against SARS-CoV-2. Biomedical Journal, 46(1):70-80. 本人為第一作者.

4.          Yi-Ju Han, Kuo-Ming Lee, Guan-Hong Wu, Yu-Nong Gong, Avijit Dutta, Shin-Ru Shih (2023, Feb). Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites. Journal of Biomedical Science, 30(1):10.

5.          Yu-An Kung^#, Kuo-Ming Lee^#, Huan-Jung Chiang^#, Sheng-Yu Huang, Chung-Jung Wu, Shin-Ru Shih (2022, Jun). Molecular Virology of SARS-CoV-2 and Related Coronaviruses. Microbiology and Molecular Biology Reviews, 86(2):e0002621. 本人為第一作者.

6.          Richard Janissen, Andrew Woodman, Djoshkun Shengjuler, Thomas Vallet, Kuo-Ming Lee, Louis Kuijpers, Ibrahim M Moustafa, Fiona Fitzgerald, Peng-Nien Huang, Angela L Perkins, Daniel A Harki, Jamie J Arnold, Belén Solano, Shin-Ru Shih, Marco Vignuzzi, Craig E Cameron, Nynke H Dekker (2021, Nov). Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses. Molecular Cell, 81(21), 4467-4480.

7.          Sheng-Yu Huang, Yu-An Kung, Peng-Nien Huang, Sheng-Yun Chang, Yu-Nong Gong, Yi-Ju Han, Huan-Jung Chiang, Kuan-Ting Liu, Kuo-Ming Lee, Chia-Yu Chang, Chia-Ching Chang, Chung-Guei Huang, Shin-Ru Shih (2021, Mar). Stability of SARS-CoV-2 Spike G614 Variant Surpasses That of the D614 Variant after Cold Storage. mSphere, 6(2), e00104-21.

8.          Yu-Nong Gong, Kuo-Chien Tsao, Mei-Jen Hsiao, Chung-Guei Huang, Peng-Nien Huang, Po-Wei Huang, Kuo-Ming Lee, Yi-Chun Liu, Shu-Li Yang, Rei-Lin Kuo, Kuan-Fu Chen, Yen-Chin Liu, Sheng-Yu Huang, Hsing-I Huang, Ming-Tsan Liu, Ji-Rong Yang, Cheng-Hsun Chiu, Cheng-Ta Yang, Guang-Wu Chen, Shin-Ru Shih (2020, Dec). SARS-CoV-2 genomic surveillance in Taiwan revealed novel ORF8-deletion mutant and clade possibly associated with infections in Middle East. Emerging Microbes & Infections, 9(1):1457-1466.

9.          Kuo-Ming Lee, Yu-Nong Gong, Shin-Ru Shih (2020, Nov). Methods for detection and study of virus-derived small RNAs produced from the intramolecular base-pairing region of the picornavirus genome. Methods, 183, 4-12. 本人為第一作者.

10.      Chung-Guei Huang^#, Kuo-Ming Lee^#, Mei-Jen Hsiao, Shu-Li Yang, Peng-Nien Huang, Yu-Nong Gong, Tzu-Hsuan Hsieh, Po-Wei Huang, Ya-Jhu Lin, Yi-Chun Liu, Kuo-Chien Tsao, Shin-Ru Shih (2020, Jul). Culture-based virus isolation to evaluate potential infectivity of clinical specimens tested for COVID-19. Journal of Clinical Microbiology, 58(8), e01068-20. 本人為第一作者.

11.      Kuo-Ming Lee^#, Chih-Ching Wu^#, Shang-En Wu, Ya-Han Lin, Li-Ting Wang, Chun-Ru Chang, Peng-Nien Huang, Shin-Ru Shih, Rei-Lin Kuo (2020, Apr). The RNA-dependent RNA polymerase of enterovirus A71 associates with ribosomal proteins and positively regulates protein translation. RNA Biology, 17(4), 608-622. 本人為第一作者.

12.      Bo-Shiun Chen, Hou-Chen Lee, Kuo-Ming Lee, Yu-Nong Gong, and Shin-Ru Shih (2020, Feb). Enterovirus and Encephalitis. Frontiers in Microbiology, 11, 261.

13.      Tzu-Wei Chuang, Chia-Chen Lu, Chun-Hao Su, Pei-Yu Wu, Sarasvathi Easwvaran, Chi-Chieh Lee, Hung-Che Kuo, Kuan-Yang Hung, Kuo-Ming Lee, Ching-Yen Tsai, Woan-Yuh Tarn (2019, Mar). The RNA Processing Factor Y14 Participates in DNA Damage Response and Repair. iScience, 13, 402-415.

14.      Andrew Woodman, Kuo-Ming Lee, Richard Janissen, Yu-Nong Gong, Nynke H. Dekker, Shin-Ru Shih, Craig E. Cameron (2019, Feb). Predicting Intraserotypic Recombination in Enterovirus 71. Journal of Virology, 93(4), e02057-18.

15.      Kuo-Ming Lee^#, Yu-Nong Gong^#, Tzu-Hsuan Hsieh, Andrew Woodman, Nynke H. Dekker, Craig E. Cameron, Shin-Ru Shih (2018, Jun). Discovery of Enterovirus A71-like nonstructural genomes in recent circulating viruses of the Enterovirus A species. Emerging Microbes & Infections, 7(1),111. 本人為第一作者.

16.      Kuo-Ming Lee^#, Chi-Jene Chen^#, and Shin-Ru Shih (2017, Jul). Regulation Mechanisms of Viral IRES-Driven Translation. Trends in Microbiology, 25(7), 546-561. 本人為第一作者.

17.      Chuan-Tien Hung, Yu-An Kung, Mei-Ling Li, Gary Brewer, Kuo-Ming Lee, Shih-Tung Liu, and Shin-Ru Shih (2016, Oct). Additive Promotion of Viral Internal Ribosome Entry Site-Mediated Translation by Far Upstream Element-Binding Protein 1 and an Enterovirus 71-Induced Cleavage Product. PLoS Pathogen, 12(10), e1005959.

18.      Rei-Lin Kuo, Zong-Hua Li, Li-Hsin Li, Kuo-Ming Lee, Ee-Hong Tam, Helene M. Liu, Hao-Ping Liu, Shin-Ru Shih, and Chih-Ching Wu (2016, May). Interactome Analysis of the NS1 Protein Encoded by Influenza A H1N1 Virus Reveals a Positive Regulatory Role of Host Protein PRP19 in Viral Replication. Journal of Proteome Research, 15(5),1639-48.

19.      Tzu-Wei Chuang, Kuo-Ming Lee, Yuan-Chao Lou, Chia-Chen Lu and Woan-Yuh Tarn (2016, Apr). A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. Journal of Biological Chemistry, 291(16), 8565-74.

20.      Tzu-Wei Chuang^#, Kou-Ming Lee^#, and Woan-Yuh Tarn (2015, Apr). Function and Pathological Implications of Exon Junction Complex Factor Y14. Biomolecules, 5(2), 343-55. 本人為第一作者.

21.      Kuo-Ming Lee and Woan-Yuh Tarn (2014, Nov). TRAP150 activates splicing in composite terminal exons. Nucleic Acids Research, 42(20),12822-32. 本人為第一作者.

22.      Kuo-Ming Lee and Woan-Yuh Tarn (2013, Mar). Coupling pre-mRNA processing to transcription on the RNA factory assembly line. RNA Biology, 10(3), 380-90. 本人為第一作者.

23.      Tzu-Wei Chuang, Wei-Lun Chang, Kuo-Ming Lee, and Woan-Yuh Tarn (2013, Jan). The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation. Molecular Biology of the Cell, 24(1), 1-13.

24.      Kuo-Ming Lee, Ia-Wen Hsu and Woan-Yuh Tarn (2010, Jun). TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation. Nucleic Acids Research, 38(10), 3340-50. 本人為第一作者.

25.      Yi-Tzang Tsai, Chen-I Lin, Hung-Kai Chen, Kuo-Ming Lee, Chia-Yi Hsu, Shun-Jen Yang, and Ning-Hsing Yeh (2008, Jul). Chromatin tethering effects of hNopp140 are involved in the spatial organization of nucleolus and the rRNA gene transcription. Journal of Biomedical Science, 15(4), 471-86.

26.      Wen-Cheng Chang, Yung-Chia Chen, Kuo-Ming Lee and Woan-Yuh Tarn (2007, Mar). Alternative splicing and bioinformatic analysis of human U12-type introns. Nucleic Acids Research, 35(6), 1833-41.

專書論文

1.          Yu-Nong Gong, Kuo-Ming Lee, Shin-Ru Shih. Evolution and Epidemiology of SARS-CoV-2 Virus. Methods in Molecular Biology (ISBN: 978-1-0716-2111-0). Humana, New York, NY: Springer Nature Group. 2022: 2452:3-18.