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Department of Medical Technology
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Hsiu-Chuan Yen

Hsiu-Chuan Yen

JobTitle： Professor

CurrentJob： Professor

E-mail： yen@mail.cgu.edu.tw

Phone： 5207

Education： Ph. D., Toxicology, University of Kentucky

Expertise： Free Radical Biology and Medicine, Mitochondrial Biology and Medicine, Toxicology, Clinical Chemistry.

Website： https://sites.google.com/view/yenlab/

Lab & Research Interest


  1. Our laboratory has established the only platform available in Taiwan to detect F2-isoprostanes, isofurans, and F4-neuroprostanes in human and mouse body fluids and mouse tissues through gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS). These markers are gold-standard markers of lipid peroxidation in vivo and we used them to study the roles of oxidative damage in various human diseases or toxicity. The research topics that have utilized this platform in collaboration with clinicians at Linkou Chang Gung Memorial Hospitals are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, immunosuppressive therapy for paraquat (an herbicide) poisoning, and chelation therapy for lead (Pb) and mercury (Hg) poisoning.
  2. We have been investigating the regulation of endogenous coenzyme Q10 levels and human PDSS and COQ genes or proteins essential for biosynthesis of coenzyme Q10 in response to oxidative stress and mitochondrial dysfunction in human cells or cybrids harboring mitochondrial DNA (mtNDA) mutations. We have then established the technique of two-dimensional blue native -polyacrylamide gel electrophoresis (2D BN-PAGE) to identify high-molecular-weight protein complexes PDSS and several COQ proteins in the mitochondria of human cells and to further explore the existence of similar protein complexes in the mitochondria of mouse cells and tissues.
  3. We have been evaluating the optimal conditions of analyzing human mtDNA mutations and copy number by Oxford Nanopore long-read next generation sequencing through the use of cybrid cells harboring pathogenic mtDNA mutations and ρ0 cells lacking mtDNA.


Publication

近年所發表期刊論文(Publications in recent years; updated on 2024/7)

1.    HC Yen*, YC Liu, CC Kan, HJ Wei, SH Lee, YH Wei, YH Feng, CW Chen, CC Huang. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochimica et Biophysica Acta - General Subjects, 1860: 1864-1876, 2016. 

2.    HC Yen*, CL Lin, BS Chen, CW Chen, KC Wei, ML Yang, JC Hsu, YH Hsu. Alterations of the levels of primary antioxidant enzymes in different grades of human astrocytoma tissues. Free Radical Research, 52: 856-871, 2018. 

3.    HC Yen*, WY Yeh, SH Lee, YH Feng, SL Yang. Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other’s stability. Biochim Biophys Acta -Bioenergetics, 1861:148192, 2020.

4.    HC Yen*, BS Chen, SL Yang, SY Wu, CW Chang, KC Wei, JC Hsu, YH Hsu, TH Yen, CL Lin*. 2022 February. Levels of coenzyme Q10 and several COQ proteins in human astrocytoma tissues are inversely correlated with malignancy. Biomolecules, 12: 336, 2022.

5. TH Yen, CW Chang, HR Tsai, JF Fu, HC Yen*. Immunosuppressive therapies attenuate paraquat-induced renal dysfunction by suppressing inflammatory responses and lipid peroxidation. Free Radic Biol Med, 191: 249-260, 2022.

6. M Kotepui*, K Kotepui, A Mahittikorn*, HJ Majima, J Tangpong, HC Yen. Association of reduced glutathione levels with Plasmodium falciparum and Plasmodium vivax malaria: a systematic review and meta‑analysis. Scientific Reports, 13: 14683, 2023.

7. D Masuda, I Nakanishi*, K Ohkubo, H Ito, K Matsumoto, H Ichikawa, M Chatatikun, WK Klangbud, M Kotepui, M Imai, F Kawakami, M Kubo, H Matsui, J Tangpong, T Ichikawa, T Ozawa, HC Yen, DK St. Clair, HP Indo*, HJ Majima*. Mitochondria play essential roles in intracellular protection against oxidative stress—Which molecules among the ROS generated in the mitochondria can escape the mitochondria and contribute to signal activation in cytosol? Biomolecules, 14: 128, 2024.

8. HC Yen*, CT Hsu, SY Wu, CC Kan, CW Chang, HM Chang, YA Chien, YH Wei, CY Wu. Alterations in coenzyme Q10 status in a cybrid line harboring the 3243A>G mutation of mitochondrial DNA is associated with abnormal mitochondrial bioenergetics and dysregulated mitochondrial biogenesis. Biochimica et Biophysica Acta - Bioenergetics, 1864: 149492, 2024.

* 負責作者(corresponding author)

其他更早論文請見實驗室網頁

(Please find other previous publications on lab webpage.)