Lab & Research Interest

1. To investigate mechanisms of antibiotics resistance and pathogenesis inStaphylococcus aureus. Staphylococcus aureus which has long been recognized as a major cause of nosocomial infections can result in sepsis and septic shock associated with vascular damage and multiple organ failure. The spread of multidrug-resistant Staphylococcus aureus has raised a serious problem in the limitation of treatment options in the clinical environment. We are interested to study the impact of environmental stress on the expression of different virulence factors in S. aureus. In addition, regulatory mechanisms for antibiotic resistance and biofilm development will also be studied.
2. To study the bacteria (S. aureus)-host interaction. Bacterial infections are the result of complex interactions between invading bacteria and host defense mechanisms. One of the aims in this laboratory will be focused on evaluating the S. aureus pathogenecity in murine models upon environmental stress.
3. Molecular Epidemiology survey. In cooperation with colleagues in ChangGung Memorial Hospital, molecular epidemiology survey for multidrug resistant pathogenic bacteria will be carried out.

Publication

1. Chen CJ, Lin MH, Shu JC, Lu JJ*. (2014 Feb). Reduced susceptibility to vancomycin in isogenic Staphylococcus aureus strains of sequence type 59: tracking evolution and identifying mutations by whole-genome sequencing. Journal of Antimicrobial Chemotherapy. 69: 349-54.
2. Kuo AJ, Su LH, Shu JC, Wang JT, Wang JH, Fung CP, Chia JH, Lu JJ, Wu TL*. (2014 Dec). National surveillance on vancomycin-resistant Enterococcus faecium in Taiwan: emergence and widespread of ST414 and a Tn1546-like element with simultaneous insertion of IS1251-like and IS1678. PLoS One. 9: e115555.
3. Shu JC, Su LH, Chiu CH, Kuo AJ, Liu TP, Lee MH, Su IN, Wu TL*.(2015 Apr). Reduced Production of OprM May Promote oprD Mutations and Lead to Imipenem Resistance in Pseudomonas aeruginosaCarrying an oprD-Group 1A Allele. Microbial Drug Resistance. 21: 149-57.
4. Lin MH#, Shu JC#, Lin LP, Chong KY, Cheng YW, Du JF, Liu ST*.(2015 Apr). Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis. PLoS One. 10: e0124216.
5. Hsu CY#, Shu JC#*, Lin MH, Chong KY, Chen CC, Wen SM, Hsieh YT, Liao WT. (2015 Aug). High glucose concentration promotes vancomycin-enhanced biofilm formation of vancomycin-non-susceptible Staphylococcus aureus in diabetic mice. PLoS One. 10:e0134852. (IF=2.806; 15/64, MULTIDISCIPLINARY SCIENCES).
6. Chen JC, Liu JH, Hsu DW, Shu JC, Chen CY, Chen CC*. (2015 Sep). Methylatable signaling helix coordinated inhibitory receiver domain in sensor kinase modulates environmental stress response in Bacillus cereus. PLoS One. 10: e0137952.
7. Atla SB, Chen YJ, Chiu HW, Chen CC, Shu JC, Chen CY*. (2016 Mar). Microbial induced synthesis of CeCO3OH and CeO2 hollow rods micro/nanostructure. Materials Letters. 167: 238-41.
8. Lee IC*, Lin WM, Shu JC, Tsai SW, Chen CH, Tsai MT. (2017 Jan). Formulation of two-layer dissolving polymeric microneedle patches for insulin transdermal delivery in diabetic mice. Journal of Biomedical Materials Research part A. 105: 84-93.
9. Chen HY, Lin MH, Chen CC, Shu JC*. (2017 Apr). The expression of fibronectin is significantly suppressed in macrophages to exert a protective effect against Staphylococcus aureus infection. BMC microbiology. 17: 92.
10. Shu JC, Kuo AJ, Su LH, Liu TP, Lee MH, Su, IN, Wu TL*. (2017 Sep). Development of carbapenem resistance in Pseudomonas aeruginosa is associated with OprD polymorphisms, particularly the amino acid substitution at codon 170. Journal of Antimicrobial Chemotherapy. 72: 2489-95.
11. Lin YH, Kang YC, Hou CH, Huang YC, Chen CJ, Shu JC, Hsieh PH, Hsiao CH*. (2017 Aug). Antibiotic susceptibility profiles of ocular and nasal flora in patients undergoing cataract surgery in Taiwan: an observational and cross-sectional study. BMJ Open. 7: e017352.
12. Lin LC#, Shu JC#, Chang SC, Ge MC, Liu TP, Chen CW, Lu JJ*.  (2017 Dec). Nucleotide sequence variations in autolysis genes of ST59 methicillin-resistant Staphylococcus aureus isolates. Microbial Drug Resistance. 23: 940-8.
13. Chen JC, Chang CF, Hsu DW, Shu JC, Chen HY, Chen CY, Lu CY, Chen CC*. (2017 Dec). Temporal regulation of σB by partner-switching mechanism at a distinct growth stage in Bacillus cereus. International Journal of Medical Microbiology. 307: 521-32.
14. Lin MH, Li CC, Shu JC, Chu HW, Liu CC, Wu CC*. (2018 Mar). Exoproteome profiling reveals the involvement of the foldase PrsA in the cell surface properties and pathogenesis of Staphylococcus aureus. Proteomics. 18: e1700195.
15. Yang CC, Sy CL, Huang YC, Shie SS, Shu JC, Hsieh PH, Hsiao CH, Chen CJ*. (2018 May). Risk factors of treatment failure and 30-day mortality in patients with bacteremia due to MRSA with reduced vancomycin susceptibility. Scientific Reports. 8: 7868.
16. Hsiao CH, Hwang YS, Chuang WY, Ma DHK, Yeh LK, Chen SY, Shu JC*. (2018 Jun). Prevalence and clinical consequences of cytomegalovirus DNA in the aqueous humour and corneal transplants. British Journal of Ophthalmology. pii: bjophthalmol-2018-312196. doi: 10.1136/bjophthalmol-2018-312196.
17. Kuo AJ, Shu JC, Liu TP, Lu JJ, Lee MH, Wu TS, Su LH, Wu TL*.(2018 Aug). Vancomycin-resistant Enterococcus faecium at a university hospital in Taiwan, 2002-2015: Fluctuation of genetic populations and emergence of a new structure type of the Tn1546-like element. Journal of Microbiology Immunology and Infection. pii: S1684-1182(18)30320-7. doi: 10.1016/j.jmii.2018.08.008.
18. Atla SB, Lin WR, Chien TC, Tseng MJ, Shu JC, Chen CC, Chen CY*. (2018 Sep). Fabrication of Fe3O4/ZnO magnetite core shell and its application in photocatalysis using sunlight. Materials Chemistry and Physics. 216: 380-6.

#Co-first author; *Corresponding author