Department of Biomedical Sciences (Master's Program in Clinical Trials Assessment)

Professional Experience

Professional Affiliations

Member of Council, Taiwan Metabolomics Society

Member of Council, Society for Free Radical Research-Taiwan

Member, Metabolomics Society

Member, Society for Free Radical Research-Asia

Patents

Prof. Cheng have five patents for Heart Failure biomarker application (US 14/631,620; PRC ZL 2015 100883856; ROC I587793; ROC I553313; PRC ZL2014 1 048247907) and one patent for Sarcopenia mortality prediction (ROC I769956).

Lab & Research Interest

As head of the Metabolomics Core Lab of the Healthy Aging Research Center at Chang Gung University, I oversee a team of experts developing and applying novel analytical methodologies for global profiling and targeted analysis of small molecules and lipids. Our favored technology platforms include nuclear magnetic resonance spectrometry (NMR), liquid chromatography-time of fly mass spectrometry (LC-TOFMS), and LC-tandem mass spectrometry (LC-MS/MS), gas chromatography spectrometry (GC-MS). Our principal application of established and developing technologies includes human biofluids, tissue extracts, animal models, and cell cultures needed to explore disease mechanisms in greater depth. We work closely with clinicians and basic science researchers at the Chang Gung system to provide innovative and reliable bioanalytical solutions for metabolomics and lipidomics.

We are most active in the areas of:

1. Analytical method development for small molecules (metabolites)

2. Metabolite assignment and identification

3. High-precision analysis demanded by large sample cohorts from molecular epidemiology studies

4. Those studies involving clinical diseases such as cardiovascular disease, Parkinson's disease, diabetes, and sarcopenia

Publications

1.          Lin Y, Wu RC, Lin YC, Huang YL, Lin CY, Lo CJ, Lu HY, Lu KY, Tsai SY, Hsieh CY, Yang LY, Cheng ML, Angel Chao, Lai CH, Gin G. (2024) Endometrial cancer risk stratification using MRI radiomics: corroborating with choline metabolism. Cancer Imaging 24(1):112.

2.          Lynn KS, Tang HY, Lo CJ, Yang CH, Tseng YT, Cheng ML. (2024) MRMQuant: Automated MRM Data Quantitation for Large-Scale Targeted Metabolomics Analysis. Analytical Chemistry 96(33): 13625-13635.

3.          Chang KH, Cheng ML, Tang HY, Lin CY, Chen CM. (2024) Dysregulation of choline metabolism and therapeutic potential of citicoline in Huntington's disease. Aging Cell ():e14302.

4.          Lee CW, Tsai HI, Hsu HM, Yu MC, Lee WC, Wang CC, Hsieh YC, Lin CY, Cheng ML, Lo CJ, Wang CT, Lin YC. (2024) Do low skeletal muscle bulk and disturbed body fat mass impact tumor recurrence in stage I/II HCC undergoing surgery? An observational cohort study. International Journal of Surgery ():10.1097/JS9.0000000000001905.

5.          Lin WC, Lo CJ, Cheng ML, Zeng RJ, Hsieh YY, Hou PR, Yeh CW, Hsieh CT, Chang MC, Liao CH. (2024) An Off-Chip Capacitance Biosensor Based on Improved Cole–Cole Model for the Detection of Trimethylamine N-Oxide in Early Cardiovascular Disease. IEEE Sensors Journal 24(7): 11515-11526.

6.          Lin WC, Hsieh YY, Ho PR, Zeng RJ, Hsieh CT, Chang MC, Yeh CW, Lo CJ, Cheng ML, Liao CH. (2024) Rapid and Direct Detection of Trimethylamine N-oxide Using an Off-Chip Capacitance Biosensor with Readout SoC for Early-Stage Thrombosis and Cardiovascular Disease. ACS Sensors 9(2):638-645.

7.          Yang CJ, Chang HC, Sung PC, Ge MC, Tang HY, Cheng ML, Cheng HT, Chou HH, Lin CY , Lin WR, Lee YS, Hsieh SY. (2024) Oral fecal transplantation enriches Lachnospiraceae and butyrate to mitigate acute liver injury. Cell Reports 43(1):113591.

8.          Liang YJ, Chiou YW, Chiu A PT, Shiao MS, Teng W, Lin CW, Cheng ML, Huang YH, Liang KH, Su CW, Lai CY, Chen CL, Wu JC. (2023) Antiviral therapy reduces hepatocellular carcinoma through suppressing hepatitis B virus replication may improve ER stress, mitochondrial and metabolic dysfunctions and decrease p62 in hybridized mice with single HBV transgene and miR-122. Journal of Medical Virology 95(12): e29325.

9.          Lo CJ, Lin CM, Fan CM, Tang HY, Liu HF, Ho HY, Cheng ML. (2023) Plasma acylcarnitine in elderly Taiwanese: as biomarkers of possible sarcopenia and sarcopenia. BMC Geriatrics 23(1):769.

10.      Chou MC, Wang YH, Chen FY, Kung CY, Wu KP, Kuo JC, Chan SJ, Cheng ML, Lin CY, Chou YC, Ho MC, Firestine S, Huang JR, Chen RH. (2023) PAICS ubiquitination recruits UBAP2 to trigger phase separation for purinosome assembly. Molecular Cell 83(22): 4123-4140.

11.      Liu FC, Cheng ML*, Lo CJ, Hsu WC, Lin G, Lin HT. (2023) Exploring the aging process of cognitively healthy adults by analyzing cerebrospinal fluid metabolomics using liquid chromatography-tandem mass spectrometry. BMC Geriatrics 23(1):217.

12.      Gau RY, Tsai HI, Yu MC, Chan KM, Lee WC, Wang HE, Wang SF, Cheng ML, Chiu CC, Chen HY, Lee CW. (2022) Laparoscopic liver resection is associated with less significant muscle loss than the conventional open approach. World Journal of Surgical Oncology 20(1): 385.

13.      Liang YJ, Chiang KM, Xiu LL, Chung CM, Lo CJ, Shiao MS, Cheng ML, Kuo CC, Yang HC, Pan WH. (2022) Pharmacometabolomic study of drug response to antihypertensive medications for hypertension marker identification in Han Chinese individuals in Taiwan. Computational and Structural Biotechnology Journal 20: 6458-6466.

14.      Wu IW, Tsai TH, Lo CJ, Chou YJ, Yeh CH, Chan YH, Chen JH, Hsu PW, Pan HC, Hsu HJ, Chen CY, Lee CC, Shyu YC, Lin CL, Cheng ML, Lai CC, Sytwu HK, Tsai TF. (2022) Discovering a trans-omics biomarker signature that predisposes high risk diabetic patients to diabetic kidney disease. NPJ Digital Medicine 5(1): 166.

15.      Wang PY, Chakraborty A, Ma HJ, Wu JW, Jang AC, Lin WC, Pi HW, Yeh CT, Cheng ML, Yu JS, Pai LM. (2022) Drosophila CTP synthase regulates collective cell migration by controlling the polarized endocytic cycle. Development 149 (16): dev200190.

16.      Chou PC, Liu CM, Weng CH, Yang KC, Cheng ML, Lin YC, Yang RB, Shyu BC, Shyue SK, Liu JD, Chen SP, Hsiao M, Hu YF. (2022) Fibroblasts Drive Metabolic Reprogramming in Pacemaker Cardiomyocytes. Circulation Research 131 (1): 6-20.

17.      Wu IW, Tsai TH, Lo CJ, Chou YJ, Yeh CH, Cheng ML, Lai CC, Sytwu HK, Tsai TF. (2022) Discovery of a Biomarker Signature That Reveals a Molecular Mechanism Underlying Diabetic Kidney Disease via Organ Cross Talk. Diabetes Care 45 (6): e102-e104.

18.      Chang KH, French IT, Liang WK, Lo YS, Wang YR, Cheng ML, Huang NE, Wu HC, Lim SN, Chen CM, Juan CH. (2022) Evaluating the Different Stages of Parkinson's Disease Using Electroencephalography With Holo-Hilbert Spectral Analysis. Frontiers in Aging Neuroscience 14: 832637.

19.      Chiu CY, Cheng ML, Chiang MH, Wang CJ, Tsai MH, Lin G. (2021) Integrated metabolic and microbial analysis reveals host-microbial interactions in IgE-mediated childhood asthma. Scientific Reports 11(1): 23407.

20.      Lin HT, Cheng ML, Lo CJ, Hsu WC, Lin G, Liu FC. (2021) ¹H NMR metabolomic profiling of human cerebrospinal fluid in aging process. American Journal of Translational Research 13(11): 12495-12508.

21.      Yeh TH, Liu HF, Chiu CC, Cheng ML, Huang GJ, Huang YC, Liu YC, Huang YZ, Lu CS, Chen YC, Chen HY, Cheng YC. (2021) PLA2G6 mutations cause motor dysfunction phenotypes of young-onset dystonia-parkinsonism type 14 and can be relieved by DHA treatment in animal models. Experimental Neurology 346:113863.

22.      Wu MJ, Chen CJ, Lin TY, Liu YY, Tseng LL, Cheng ML, Chuu CP, Tsai HK, Kuo WL, Kung HJ, Wang WC. (2021) Targeting KDM4B that coactivates c-Myc-regulated metabolism to suppress tumor growth in castration-resistant prostate cancer. Theranostics 11(16): 7779-7796.

23.      Hsu SC, Chen CL, Cheng ML, Chu CY, Changou CA, Yu YL, Yeh SD, Kuo TC, Kuo CC, Chuu CP, Li CF, Wang LH, Chen HW, Yen Y, Ann DK, Wang HJ, Kung HJ. (2021) Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes. Theranostics 11(15): 7527-7545.

24.      Huang CF, Chen A, Lin SY, Cheng ML, Shiao MS, Mao TY. (2021) A metabolomics approach to investigate the proceedings of mitochondrial dysfunction in rats from prediabetes to diabetes. Saudi Journal of Biological Sciences 28(8): 4762-4769.

25.      Ma H, Zhang F, Zhou L, Cao T, Sun D, Wen S, Zhu J, Xiong Z, Tsau MT, Cheng ML, Hung LM, Zhou Y, Li Q. (2021) c-Src facilitates tumorigenesis by phosphorylating and activating G6PD. Oncogene 40(14): 2567-2580.

26.      Tsai HI, Lo CJ, Lee CW, Lin JR, Lee WC, Ho HY, Tsai CY, Cheng ML^#, Yu HP. (2021) A panel of biomarkers in the prediction for early allograft dysfunction and mortality after living donor liver transplantation. American Journal of Translational Research 13(1): 372-382.

27.      Cheng CW, Liu MH, Tang HY, Cheng ML, Wang CH. (2021) Factors associated with elevated plasma phenylalanine in patients with heart failure. Amino Acids 53(2): 149-157.

28.      Lo CJ, Ko YS, Chang SW, Tang HY, Huang CY, Huang YC, Ho HY, Lin CM, Cheng ML. (2020) Metabolic signatures of muscle mass loss in an elderly Taiwanese population. Aging 13(1):944-956.

29.      Chen WS, Liu MH, Cheng ML, Wang CH. (2020) Decreases in Circulating Concentrations of Short-Chain Acylcarnitines are Associated with Systolic Function Improvement After Decompensated Heart Failure. International Heart Journal 61(5):1014-1021.

30.      Huang JP, Cheng ML, Wang CH, Huang SS, Hsieh PS, Chang CC, Kuo CY, Chen KH, Hung LM. (2020) Therapeutic potential of cPLA2 inhibitor to counteract dilated-cardiomyopathy in cholesterol-treated H9C2 cardiomyocyte and MUNO rat. Pharmacological Research 160: 105201.

31.      Chang-Chien J, Huang HY, Tsai HJ, Lo CJ, Lin WC, Tseng YL, Wang SL, Ho HY, Cheng ML^#, Yao TC. (2020) Metabolomic differences of exhaled breath condensate among children with and without asthma. Pediatric Allergy and Immunology 32(2): 264-272.

32.      Liang KH, Cheng ML^*, Lo CJ, Lin YH, Lai MW, Lin WR, Yeh CT. (2020) Plasma phenylalanine and glutamine concentrations correlate with subsequent hepatocellular carcinoma occurrence in liver cirrhosis patients: an exploratory study. Scientific Reports 10(1):10926.

33.      Chiu CY, Cheng ML, Chiang MH, Wang CJ, Tsai MH, Lin G. (2020) Metabolomic Analysis Reveals Distinct Profiles in the Plasma and Urine Associated with IgE Reactions in Childhood Asthma. Journal of Clinical Medicine 9(3): E887.

34.      Ma H, Zhang J, Zhou L, Wen S, Tang HY, Jiang B, Zhang F, Suleman M, Sun D, Chen A, Zhao W, Lin F, Tsau MT, Shih LM, Xie C, Li X, Lin D, Hung LM, Cheng ML^#, Li Q. (2020) c-Src Promotes Tumorigenesis and Tumor Progression by Activating PFKFB3. Cell Reports 30(12): 4235-4249.

35.      Weng WC, Huang WY, Tang HY, Cheng ML, Chen KH. (2019) The Differences of Serum Metabolites Between Patients With Early-Stage Alzheimer's Disease and Mild Cognitive Impairment. Frontiers in Neurology 10: 1223.

36.      Cheng SC, Chen K, Chiu CY, Lu KY, Lu HY, Chiang MH, Tsai CK, Lo CJ, Cheng ML, Chang TC, Lin G. (2019) Metabolomic biomarkers in cervicovaginal fluid for detecting endometrial cancer through nuclear magnetic resonance spectroscopy. Metabolomics 15(11): 146.

37.      Lin HT, Cheng ML^*, Lo CJ, Lin G, Lin SF, Yeh JT, Ho HY, Lin JR, Liu FC. (2019) 1H Nuclear Magnetic Resonance (NMR)-Based Cerebrospinal Fluid and Plasma Metabolomic Analysis in Type 2 Diabetic Patients and Risk Prediction for Diabetic Microangiopathy. Journal of Clinical Medicine 8(6): E874.

38.      Wang CH, Cheng ML, Liu MH, Fu TC. (2019) Amino Acid-Based Metabolic Profile Provides Functional Assessment and Prognostic Value for Heart Failure Outpatients. Disease Markers 2019: 8632726.

39.      Lee TH, Cheng ML^*, Shiao MS, Lin CN. (2019) Metabolomics study in severe extracranial carotid artery stenosis. BMC Neurology 19(1): 138.

40.      Chiu CY, Cheng ML, Chiang MH, Kuo YL, Tsai MH, Chiu CC, Lin G. (2019) Gut microbial-derived butyrate is inversely associated with IgE responses to allergens in childhood asthma. Pediatric Allergy and Immunology 30(7): 689-697.

41.      Huang SS, Lin JY, Chen WS, Liu MH, Cheng CW, Cheng ML, Wang CH. (2019) Phenylalanine and leucine-defined metabolic types identify high mortality risk in patients with severe infection. International Journal of Infectious Diseases 85: 143-149.

42.      Hung CY, Yeh TS, Tsai CK, Wu RC, Lai YC, Chiang MH, Lu KY, Lin CN, Cheng ML, Lin G. (2019) Glycerophospholipids pathways and chromosomal instability in gastric cancer: Global lipidomics analysis. World Journal of Gastrointestinal Oncology 11(3):181-194.

43.      Yang CH, Liang CT, Jiang ST, Chen KH, Yang CC, Cheng ML, Ho HY. (2019) A Novel Murine Model Expressing a Chimeric mSCARB2/hSCARB2 Receptor Is Highly Susceptible to Oral Infection with Clinical Isolates of Enterovirus 71. Journal of Virology 93(11):e00183-19.

44.      Chiu CY, Cheng ML, Wong KS, Lai SH, Chiang MH, Tsai MH, Lin G. (2019) Metabolomics Reveals Anaerobic Bacterial Fermentation and Hypoxanthine Accumulation for Fibrinous Pleural Effusions in Children with Pneumonia. Journal of proteome research 18(3):1248-1254.

45.      Cheng ML, Lin JF, Huang CY, Li GJ, Shih LM, Chiu DT, Ho HY. (2019) Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress. Oxidative Medicine and Cellular Longevity 2019:5913635.

46.      Chen IC, Chang CN, Chen WL, Lin TH, Chao CY, Lin CH, Lin HY, Cheng ML, Chiang MC, Lin JY, Wu YR, Lee-Chen GJ, Chen CM. (2019) Targeting Ubiquitin Proteasome Pathway with Traditional Chinese Medicine for Treatment of Spinocerebellar Ataxia Type 3. The American Journal of Chinese Medicine 47(1): 63-95.

47.      Lynn KS, Cheng ML, Yang HC, Liang YJ, Kang MJ, Chen FL, Shiao MS, Pan WH. (2019) Vegetable signatures derived from human urinary metabolomic data in controlled feeding studies. Journal of proteome research 18(1): 159-168.

48.      Wang CH, Cheng ML, Liu MH. (2018) Amino Acid-Based Metabolic Panel Provides Robust Prognostic Value Additive to B-Natriuretic Peptide and Traditional Risk Factors in Heart Failure. Disease Markers 2018:3784589.

49.      Wang CH, Cheng ML, Liu MH. (2018) Simplified plasma essential amino acid-based profiling provides metabolic information and prognostic value additive to traditional risk factors in heart failure. Amino Acids 50(12):1739-1748.

50.      Tsai CK, Yeh TS, Wu RC, Lai YC, Chiang MH, Lu KY, Hung CY, Ho HY, Cheng ML, Lin G. (2018) Metabolomic alterations and chromosomal instability status in gastric cancer. World Journal of Gastroenterology 24(33):3760-3769.

51.      Lin WC, Chakraborty A, Huang SC, Wang PY, Hsieh YJ, Chien KY, Lee YH, Chang CC, Tang HY, Lin YT, Tung CS, Luo JD, Chen TW, Lin TY, Cheng ML, Chen YT, Yeh CT, Liu JL, Sung LY, Shiao MS, Yu JS, Chang YS, Pai LM. (2018) Histidine-Dependent Protein Methylation Is Required for Compartmentalization of CTP Synthase. Cell Reports 24(10):2733-2745.

52.      Chung YH, Lu KY, Chiu SC, Lo CJ, Hung LM, Huang JP, Cheng ML, Wang CH, Tsai CK, Lin YC, Chang SH, Lin G. (2018) Early Imaging Biomarker of Myocardial Glucose Adaptations in High-Fat-Diet-Induced Insulin Resistance Model by Using 18F-FDG PET and [U-13C]glucose Nuclear Magnetic Resonance Tracer. Contrast Media & Molecular Imaging 2018:8751267.

53.      Chen KH, Chen YL, Tang HY, Hung CC, Yen TH, Cheng ML, Shiao MS, Chen JK. (2018) Dietary Leucine Supplement Ameliorates Hepatic Steatosis and Diabetic Nephropathy in db/db Mice. International Journal of Molecular Sciences 19(7):E1921.

54.      Chang KH, Cheng ML, Chiang MC, Chen CM. (2018) Lipophilic antioxidants in neurodegenerative diseases. Clinica Chimica Acta 485:79-87.

55.      Chiu CY, Lin G, Cheng ML, Chiang MH, Tsai MH, Su KW, Hua MC, Liao SL, Lai SH, Yao TC, Yeh KW, Huang JL. (2018) Longitudinal urinary metabolomic profiling reveals metabolites for asthma development in early childhood. Pediatric Allergy and Immunology 29(5): 496-503.

56.      Chang SW, Cheng ML, Shiao MS, Yeh CT, Wang CH, Fan CM, Chiu CT, Chang ML. (2018) Recovery of lipid metabolic alterations in hepatitis C patients after viral clearance: Incomplete restoration with accelerated ω-oxidation. Journal of Clinical Lipidology 12(3):756-766.

57.      Chang KH, Cheng ML^*, Tang HY, Huang CY, Wu YR, Chen CM. (2018) Alternations of Metabolic Profile and Kynurenine Metabolism in the Plasma of Parkinson’s Disease. Molecular Neurobiology 55(8):6319-6328.

58.      Tang HY, Wang CH, Ho HY, Wu PT, Hung CL, Huang CY, Wu PR, Yeh YH, Cheng ML. (2018) Lipidomics reveals accumulation of the oxidized cholesterol in erythrocytes of heart failure patients. Redox Biology 14:499-508.

59.      Sun CY, Cheng ML, Pan HC, Lee JH, Lee CC. (2017) Protein-bound uremic toxins impaired mitochondrial dynamics and functions. Oncotarget 8(44):77722-77733.

60.      Cheng WH, Huang KY, Huang PJ, Lee CC, Yeh YM, Ku FM, Lin R, Cheng ML, Chiu CH, Tang P. (2017) γ-Carboxymuconolactone decarboxylase: a novel cell cycle-related basal body protein in the early branching eukaryote Trichomonas vaginalis. Parasites & Vectors 10(1):443.

61.      Chen SG, Leu YL, Cheng ML, Ting SC, Liu CC, Wang SD, Yang CH, Hung CY, Sakurai H, Chen KH, Ho HY. (2017) Anti-enterovirus 71 activities of Melissa officinalis extract and its biologically active constituent rosmarinic acid. Scientific Reports 7(1):12264.

62.      Tang HY, Chiu DT, Lin JF, Huang CY, Chang KH, Lyu RK, Ro LS, Kuo HC, Cheng ML^#, Chen CM. (2017) Disturbance of Plasma Lipid Metabolic Profile in Guillain-Barre Syndrome. Scientific Reports 7(1):8140.

63.      Lin YH, Wu MH, Huang YH, Yeh CT, Cheng ML, Chi HC, Tsai CY, Chung IH, Chen CY, Lin KH. (2017) Taurine upregulated gene 1 functions as a master regulator to coordinate glycolysis and metastasis in hepatocellular carcinoma. Hepatology 67(1): 188-203.

64.      Wu HQ, Cheng ML^*, Lai JM, Wu HH, Chen MC, Liu WH, Wu WH, Chang PM, Huang CF, Tsou AP, Shiao MS, Wang FS. (2017) Flux balance analysis predicts Warburg-like effects of mouse hepatocyte deficient in miR-122a. PLOS Computational Biology 13(7):e1005618.

65.      Ho HY, Lin YT, Lin G, Wu PR, Cheng ML. (2017) Nicotinamide nucleotide transhydrogenase (NNT) deficiency dysregulates mitochondrial retrograde signaling and impedes proliferation. Redox Biology 12:916-928.

66.      Tang HY, Ho HY, Chiu DT, Huang CY, Cheng ML^#, Chen CM. (2017) Alterations of plasma concentrations of lipophilic antioxidants are associated with Guillain-Barre syndrome. Clinica Chimica Acta 470:75-80.

67.      Chen SG, Cheng ML, Chen KH, Horng JT, Liu CC, Wang SM, Sakurai H, Leu YL, Wang SD, Ho HY. (2017) Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. Scientific Reports 7(1):935.

68.      Wang CH, Cheng ML, Liu MH, Kuo LT, Shiao MS. (2017) Metabolic profile provides prognostic value better than galectin-3 in patients with heart failure. Journal of Cardiology 70(1):92-98.

69.      Chen TL, Yang HC, Hung CY, Ou MH, Pan YY, Cheng ML, Stern A, Lo SJ, Chiu DT. (2017) Impaired embryonic development in glucose-6-phosphate dehydrogenase-deficient Caenorhabditis elegans due to abnormal redox homeostasis induced activation of calcium-independent phospholipase and alteration of glycerophospholipid metabolism. Cell Death and Disease 8(1):e2545.

70.      Huang JP, Cheng ML, Hung CY, Wang CH, Hsieh PS, Shiao MS, Chen JK, Li DE, Hung LM. (2017) Docosapentaenoic acid and docosahexaenoic acid are positively associated with insulin sensitivity in rats fed high-fat and high-fructose diets. Journal of Diabetes 9(10):936-946.

71.      Cheng ML, Chi LM, Wu PR, Ho HY (2016) Dehydroepiandrosterone-induced changes in mitochondrial proteins contribute to phenotypic alterations in hepatoma cells. Biochemical Pharmacology 117:20–34.

72.      Huang JP, Cheng ML*, Wang CH, Shiao MS, Chen JK, Hung LM. (2016) High-fructose and high-fat feeding correspondingly lead to the development of lysoPC-associated apoptotic cardiomyopathy and adrenergic signaling-related cardiac hypertrophy. International Journal of Cardiology 215:65-76

73.      Cheng ML, Chang KH, Wu YR, Chen CM. (2016) Metabolic disturbances in plasma as biomarkers for Huntington's disease. Journal of Nutritional Biochemistry 31:38-44.

74.      Chiu CY, Lin G, Cheng ML, Chiang MH, Tsai MH, Lai SH, Wong KS, Hsieh SY. (2016) Metabolomic Profiling of Infectious Parapneumonic Effusions Reveals Biomarkers for Guiding Management of Children with Streptococcus pneumoniae Pneumonia. Scientific Reports 6:24930.

75.      Martel J, Wu CY, Hung CY, Wong TY, Cheng AJ, Cheng ML, Shiao MS, Young JD. (2016) Fatty acids and small organic compounds bind to mineralo-organic nanoparticles derived from human body fluids as revealed by metabolomic analysis. Nanoscale 8(10):5537-45.

76.      Chiu CY, Yeh KW, Lin G, Chiang MH, Yang SC, Chao WJ, Yao TC, Tsai MH, Hua MC, Liao SL, Lai SH, Cheng ML#, Huang JL. (2016) Metabolomics Reveals Dynamic Metabolic Changes Associated with Age in Early Childhood. PLoS ONE 11(2): e0149823

77.      Tsai WC, Yu TY, Lin LP, Cheng ML, Chen CL, Pang JS. (2016) Prevention of simvastatin-induced inhibition of tendon cell proliferation and cell cycle progression by geranylgeranyl pyrophosphate. Toxicological Sciences 149(2):326-34.

78.      Wang PY, Lin WC, Tsai YC, Cheng ML, Lin YH, Tseng SH, Chakraborty A, Pai LM. (2015) Regulation of CTP Synthase Filament Formation During DNA Endoreplication in Drosophila. Genetics 201(4):1511-23.

79.      Wu YH, Chiu DT, Lin HR, Tang HY, Cheng ML, Ho HY (2015) Glucose-6-Phosphate Dehydrogenase Enhances Antiviral Response through Downregulation of NADPH Sensor HSCARG and Upregulation of NF-κB Signaling. Viruses 7(12), 6689-6706.

80.      Wang CH, Cheng ML#, Liu MH, Shiao MS, Hsu KH, Huang YY, Lin CC, Lin JF. (2015) Increased p-cresyl sulfate level is independently associated with poor outcomes in patients with heart failure. Heart and Vessels 7:1100-8

81.      Yeh YH, Kuo CT, Chang GJ, Chen YH, Lai YJ, Cheng ML, Chen WJ. (2015) Rosuvastatin suppresses atrial tachycardia-induced cellular remodeling via Akt/Nrf2/heme oxygenase-1 pathway. Journal of Molecular and Cellular Cardiology 82:84-92.

82.      Cheng ML, Wang CH, Shiao MS, Liu MH, Huang YY, Huang CY, Mao CT, Lin JF, Ho HY, Yang NI. (2015) Metabolic Disturbances Identified in Plasma Are Associated With Outcomes in Patients With Heart Failure: Diagnostic and Prognostic Value of Metabolomics. Journal of The American College of Cardiology 65(15):1509-20 

83.      Yang HC, Cheng ML*, Hua YS, Wu YH, Lin HR, Liu HY, Ho HY, Chiu DT. (2015) Glucose 6-phosphate dehydrogenase knockdown enhances IL-8 expression in HepG2 cells via oxidative stress and NF-κB signaling pathway. Journal of Inflammation-London 12:34

84.      Tang HY, Ho HY, Wu PR, Chen SH, Kuypers FA, Cheng ML*^#, Chiu DT. (2015) Inability to Maintain GSH Pool in G6PD-Deficient Red Cells Causes Futile AMPK Activation and Irreversible Metabolic Disturbance. Antioxidants & Redox Signaling 22(9):744-59 (*Co-first author paper) (#Co-corresponding)

85.      Lynn KS, Cheng ML, Chen YR, Hsu C, Chen A, Lih TM, Chang HY, Huang CJ, Shiao MS, Pan WH, Sung TY, Hsu WL. (2015) Metabolite identification for mass spectrometry-based metabolomics using multiple types of correlated ion information. Analytical Chemistry 87(4):2143-51.

86.      Cheng ML, Weng SF, Kuo CH, Ho HY. (2014) Enterovirus 71 Induces Mitochondrial Reactive Oxygen Species Generation That is Required for Efficient Replication. PLoS One 9(11):e113234

87.      Ho HY, Cheng ML*, Chiu DT. (2014) Glucose-6-phosphate dehydrogenase-beyond the realm of red cell biology. Free Radical Research 48(9):1028-48 (*Co-first author paper)

88.      Chang ML, Tsou YK, Hu TH, Lin CH, Lin WR, Sung CM, Chen TH, Cheng ML, Chang KC, Chiu CT, Yeh CT, Pang JH, Shiao MS. (2014) Distinct patterns of the lipid alterations between genotype 1 and 2 chronic hepatitis C patients after viral clearance. PLoS One 9(8):e104783

89.      Chou HY, Lin YH, Shiu GL, Tang HY, Cheng ML, Shiao MS, Pai LM. (2014) ADI1, a methionine salvage pathway enzyme, is required for Drosophila fecundity. Journal of Biomedical Science 21:64

90.      Chen CM, Weng YT, Chen WL, Lin TH, Chao CY, Lin CH, Chen IC, Lee LC, Lin HY, Wu YR, Chen YC, Chang KH, Tang HY, Cheng ML, Lee-Chen GJ, Lin JY. (2014) Aqueous extract of Glycyrrhiza inflata inhibits aggregation by upregulating PPARGC1A and NFE2L2–ARE pathways in cell models of spinocerebellar ataxia 3. Free Radical Biology & Medicine 71:339–50

91.      Chow SE, Kao CH, Liu YT, Cheng ML, Yang YW, Huang YK, Hsu CC, Wang JS.(2014) Resveratrol induced ER expansion and ER caspase-mediated apoptosis in human nasopharyngeal carcinoma cells. Apoptosis. 19(3):527-41

92.      Cheng ML, Ho HY, Lin HY, Lai YC, Chiu DTY (2013) Effective NET Formation in Neutrophils from Individuals with G6PD Taiwan-Hakka is Associated with Enhanced NADP+ Biosynthesis. Free Radical Research 47: 699-709

93.      Lin HR, Wu CC, Wu YH, Hsu CW, Cheng ML#, Chiu DTY (2013) Proteome-wide dysregulation by G6PD reveals a novel protective role for G6PD in aflatoxin B1-mediated cytotoxicity.  Journal of proteome research 12:3434-48

94.      Huang CF, Cheng ML, Fan CM, Hong CY, Shiao MS (2013) Nicotinuric Acid: A Potential Marker of Metabolic Syndrome through a Metabolomics-Based Approach. Diabetes Care 36(6):1729-1731 

95.      Wu TC, Chen HT, Chang HY, Yang CY, Hsiao MC, Cheng ML, Chen JC. (2013) Mineralocorticoid receptor antagonist spironolactone prevents chronic corticosterone induced depression-like behavior. Psychoneuroendocrinology 38(6):871-883

96.      Yang HC, Chen TL, Wu YH, Cheng KP, Lin YH, Cheng ML, Ho HY, Lo SJ, Chiu DTY (2013) Glucose 6-phosphate dehydrogenase deficiency enhances germ cell apoptosis and causes defective embryogenesis in Caenorhabditis elegans. Cell Death & Disease. 4:e616

97.      Ho HY, Cheng ML*, Shiao MS, Chiu DTY. (2013) Characterization of Global Metabolic Responses of G6PD-Deficient Hepatoma Cells to Diamide-Induced Oxidative Stress. Free Radical Biology & Medicine 54:71-84

98.      Chen YC, Chen CM, Liu JL, Chen ST, Cheng ML#, Chiu DTY. (2011) Oxidative markers in spontaneous intracerebral hemorrhage: leukocyte 8-hydroxy-2'-deoxyguanosine as an independent predictor of the 30-day outcome. Journal of Neurosurgery 115:1184-1190

99.      Chen KH, Cheng ML, Jing YH, Chiu DTY, Shiao MS, Chen JK. (2011) Resveratrol ameliorates metabolic disorders and muscle wasting in streptozotocin-induced diabetic rats. American Journal of Physiology-Endocrinology and Metabolism 301:E853-E863 

100.  Cheng ML, Shiao MS, Chiu DTY, Weng SF, Tang HY, Ho HY (2011)Biochemical disorders associated with antiproliferative effect of dehydroepiandrosterone in hepatoma cells as revealed by LC-based metabolomics. Biochemical Pharmacology 82:1549-1561

101.  Lin YS, Chen CM, Soong BW, Wu YR, Chen HM, Yeh WY, Wu DR, Lin YJ, Poon PW, Cheng ML, Wang CH, Chern Y (2011)Dysregulated brain creatine kinase is associated with hearing impairment in mouse models of Huntington disease. Journal of Clinical Investigation 121:1519-1523

102.  Yang HC, Cheng ML*, Ho HY, Chiu DTY (2011)The microbicidal and cytoregulatory roles of NADPH oxidases. Microbes and Infection 13:109-120

103.  Lin CJ, Ho HY, Cheng ML, Cheng TH. Yu JS, Chiu DTY (2010) Impaired dephosphorylation renders G6PD-knockdown HepG2 cells more susceptible to H2O2-induced apoptosis. Free Radical Biology & Medicine  49:361-373

104.  Wang JS, Cheng ML, Yen HC, Lou BS, Liu HC (2009) Vitamin e suppresses enhancemnt of factor VIII-dependent thrombin generation by systemic hypoxia.  Stroke 40:656-659

105.  Ho HY, Cheng ML*, Weng SF, Leu YL, Chiu DTY (2009) Antiviral effect of epigallocatechin gallate on enterovirus 71.  Journal of Agricultural and Food Chemistry 57:6140-6147

106.  Gao LP, Cheng ML*, Chou HJ, Yang YH, Ho HY, Chiu DTY (2009) Ineffective GSH Regeneration Enhances G6PD Knockdown Hep G2 Cells to Diamide Induced-Oxidative Damage.  Free Radical Biology & Medicine 47:529-535

107.  Wu YH, Cheng ML, Ho HY, Chiu DTY, Wang TC (2009) Telomerase prevents accelerated senescence in glucose-6-phosphate dehydrogenase (G6PD) -deficient human fibroblasts.  Journal of Biomedical Science 16:18

108.  Cheng ML, Chen CM, Li JM, Ho HY, Chiu DTY (2009) Effect of Acute Myocardial Infarction on Erythrocytic Glutathione Peroxidase 1 Activity and Plasma Vitamin E Levels.  American Journal of Cardiology 103:471-475

109.  Chen CM, Liu JL, Wu YR, Chen YC, Cheng ML^#, Chiu DTY (2009) Increased oxidative damage in peripheral blood correlates with severity of Parkinson's disease.  Neurobiology of Disease 33:429-435

110.  Ho HY, Cheng ML, Chen CM, Gu PW, Wang YL, Li JM, Chiu DTY (2008) Oxidative damage markers and antioxidants in patients with acute myocardial infarction and their clinical significance.  BioFactors 34:135-145

111.  Cheng ML, Ho HY, Lin JF, Chen YC, Chan EC, Chiu DTY(2008) Clinical Relevance of plasma homocysteine levels in Taiwanese patients with coronary artery disease.  BioFactors 34:125-134

112.  Ho HY, Cheng ML*, Chiu HY, Weng SF and Chiu DTY (2008) Dehydroepiandrosterone induces growth arrest of hepatoma cells via alteration of mitochondrial gene expression and function.  International Journal of Oncology 33:969-977

113.  Ho HY, Cheng ML*, Weng SF, Chang Lo, Yeh TT, Shih SR Chiu DTY (2008) Glucose-6-phosphate dehydrogenase Deficiency Enhances Enterovirus 71 Infection.  Journal of General Virology 89:2080-2089

114.  Cheng ML, Chen CM, Gu PW, Ho HY, Chiu DTY(2008) Elevated levels of myeloperoxidase, white blood cell count and 3-chlorotyrosine in Taiwanese patients with acute myocardial infarction.   Clinical Biochemistry 41:554-560

115.  Wu YH, Tseng CP, Cheng ML, Ho HY, Shih SR, Chiu DTY (2008) Glucose-6-Phosphate Dehydrogenase Deficiency Enhances Human Coronavirus 229E Infection. Journal of Infectious Diseases 197:812-816

116.  Tsai SY, Sun NK, Lu HP, Cheng ML, Chao CC (2007) Involvement of reactive oxygen species in multidrug resistance of a vincristine-selected lymphoblastoma.  Cancer Science 98:1206-1214

117.  Chen CM, Wu YR, Cheng ML, Liu JL, Lee YM, Lee PW, Soong BW, Chiu DTY (2007) Increased oxidative damage and mitochondrial abnormalities in the peripheral blood of Huntington's disease patients.  Biochemical and Biophysical Research Communications  359:335-340

118.  Wang JS, Lin HY, Cheng ML, Wong MK (2007) Chronic Intermittent Hypoxia Modulates Eosinophil/Neutrophil-Platelet Aggregation and Inflammatory Cytokine Secretion Caused by Strenuous Exercise in Males.  Journal of Applied Physiology 103:305-314

119.  Ho HY, Cheng ML, Chiu DTY (2007) Glucose-6-Phosphate Dehydrogenase -From Oxidative Stress to Cellular Functions and Degenerative Diseases.  Redox Report 12:109-118

120.  Ho HY, Cheng ML*, Cheng PF, Chiu DTY (2007) Low Oxygen Tension Alleviates Oxidative Damage and Delays Cellular Senescence in G6PD-Deficient Cells.  Free Radical Research 41:571-579

121.  Ho HY, Cheng ML*, Wang YH, Chiu DTY (2006) Flow cytometry for Assessment of the Efficacy of siRNA.  Cytometry Part A 69:1054-1061

122.  Ho HY, Wei TT, Cheng ML^#, Chiu DTY (2006) Green tea polyphenol epigallocatechin-3- gallate protects cells against peroxynitrite-induced cytotoxicity: Modulatory effect of cellular G6PD status.  Journal of Agricultural and Food Chemistry 54:1638-1645

123.  Ho HY, Cheng ML, Chiu DTY (2005) G6PD- an old bottle with new wine.  Chang Gung Medical Journal 28:606-612

124.  Cheng ML, Ho HY, Tseng HC, Lee CH, Shih LY, Chiu DTY (2005) Antioxidant deficit and enhanced susceptibility to oxidative damage in individuals with different forms of alpha-thalassaemia.  British Journal of Haematology 128:119-127

125.  Hsu YP, Chen RJ, Fang JF, Lin BC, Huang TL, Cheng ML, Chiu DTY, Tsay PK (2004) Increased susceptibility to oxidant injury in hepatocytes from rats with intra-abdominal hypertension.  Journal of Trauma-Injury Infection and Critical Care 57:569-575

126.  Cheng ML, Ho HY, Wu YH, Chiu DTY (2004) Glucose-6-phosphate dehydrogenase (G6PD)- deficient cells show an increased propensity to oxidant-induced senescence.  Free Radical Biology & Medicine 36:580-591

127.  Cheng ML, Ho HY, Huang YW, Lu FJ and Chiu DTY (2003) Humic acid induces oxidative DNA damage, growth retardation and apoptosis in human primary fibroblasts.  Experimental Biology and Medicine 228:413-423

128.  Cheng ML, Ho HY, Liang CM, Chou YH, Stern A, Lu FJ, and Chiu DTY (2000) Cellular glucose-6-phosphate dehydrogenase (G6PD) status modulates the effects of nitric oxide (NO) on human foreskin fibroblasts.  FEBS Letters 475:257-262

129.  Ho HY, Cheng ML*, Liang CM, Lu FJ, Chou YH, Stern A, and Chiu DTY (2000) Enhanced oxidative stress and accelerated cellular senescence in glucose-6-phosphate dehydrogenase (G6PD)-deficient human fibroblasts.  Free Radical Biology & Medicine 29:156-169

130.  Lin MT, Su WC, Cheng ML, Cheng KS, Chang WC, Wing, LYC, Jen CJ, Wu HL (1999) The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture.  Journal of Biomedical Science 6:260-268

131.  Cheng ML, Ho HY, Chiu DTY, and Lu FJ (1999) Humic acid-mediated oxidative damages to human erythrocytes: a possible mechanism leading to anemia in Blackfoot disease.  Free Radical Biology & Medicine 27:470-474

132.  Cheng ML, Liu TZ, Lu FJ, Chiu DTY (1999) Simultaneous detection of vitamin C and uric acid capillary electrophoresis in plasma of diabetes and in aqueous humor in acute anterior uveitis.  Clinical Biochemistry 32:473-476

133.  Lin YS, Kao SF, Jan MS, Cheng ML, Wing LY, Chang WC, Lei HY, Lin MT (1995) Changes of protein kinase C subspecies in staphylococcal enterotoxin-B-induced thymocyte apoptosis.  Biochemical and Biophysical Research Communications 213:1132-9

(*co-first author ; ^#co-corresponding author)