Peng-Nien Huang
JobTitle： Assistant Professor
CurrentJob： Assistant Professor
E-mail： mark1005@gmail.com/mark1005@mail.cgu.edu.tw
Phone： 3723
Education： Ph.D. Graduate Institute of Biomedical Sciences, Division of Biotechnology, Chang Gung University, Taiwan.
Expertise： Molecular virology, Laboratory Animal Science, Vaccinology
Lab & Research Interest
1. Our lab focuses on studying viral replication mechanisms in hosts using animal models. We have established models for enterovirus, influenza, and SARS-CoV-2 infections, providing a natural setting to observe virus-host interactions. These models aid in understanding viral behavior and developing antiviral drugs and vaccines by evaluating their safety and efficacy.2. We have developed methods to study the relationship between gut microbiota and intestinal RNA virus infections using antibiotic treatments and fecal microbiota transplantation. Through multi-omics analysis, we investigate how gut microbiota influences viral infections and identify potential antiviral bacterial components.
3. Our BSL-3 lab operates under strict biosafety protocols to study infectious pathogens. We provide advanced research services and collaborate with industry partners to develop antiviral drugs, vaccines, and diagnostic tools. Our lab also trains personnel in high-containment laboratory practices, supporting public health efforts and research advancements.
Publications
1. Chen TC, Liu SC, Huang PN, Chang HY, Chern JH, Shih SR. Antiviral activity of pyridyl imidazolidinones against enterovirus 71 variants. J Biomed Sci. 2008 May;15(3):291-300. doi: 10.1007/s11373-007-9228-5. Epub 2008 Jan 15. PubMed PMID: 18196474.
2. Lin JY, Li ML, Huang PN, Chien KY, Horng JT, Shih SR. Heterogeneous nuclear ribonuclear protein K interacts with the enterovirus 71 5' untranslated region and participates in virus replication. J Gen Virol. 2008 Oct;89(Pt 10):2540-2549. doi: 10.1099/vir.0.2008/003673-0. PubMed PMID: 18796723.
3. Chen TC, Weng KF, Chang SC, Lin JY, Huang PN, Shih SR. Development of antiviral agents for enteroviruses. J Antimicrob Chemother. 2008 Dec;62(6):1169-73. doi: 10.1093/jac/dkn424. Epub 2008 Oct 18. Review. PubMed PMID: 18931391.
4. Tsao KC, Huang CG, Huang YL, Chen FC, Huang PN, Huang YC, Lin TY, Shih SR, Chang SC. Epidemiologic features and virus isolation of enteroviruses in Northern Taiwan during 2000-2008. J Virol Methods. 2010 May;165(2):330-2. doi: 10.1016/j.jviromet.2010.03.001. Epub 2010 Mar 7. PubMed PMID: 20214926.
5. Weng KF, Chen LL, Huang PN, Shih SR. Neural pathogenesis of enterovirus 71 infection. Microbes Infect. 2010 Jul;12(7):505-10. doi: 10.1016/j.micinf.2010.03.006. Epub 2010 Mar 27. Review. PubMed PMID: 20348010.
6. Huang PN, Lin JY, Locker N, Kung YA, Hung CT, Lin JY, Huang HI, Li ML, Shih SR. Far upstream element binding protein 1 binds the internal ribosomal entry site of enterovirus 71 and enhances viral translation and viral growth. Nucleic Acids Res. 2011 Dec;39(22):9633-48. doi: 10.1093/nar/gkr682. Epub 2011 Aug 31. PubMed PMID: 21880596; PubMed Central PMCID: PMC3239202.
7. Huang CH, Chen CJ, Yen CT, Yu CP, Huang PN, Kuo RL, Lin SJ, Chang CK, Shih SR. Caspase-1 deficient mice are more susceptible to influenza A virus infection with PA variation. J Infect Dis. 2013 Dec 1;208(11):1898-905. doi: 10.1093/infdis/jit381. Epub 2013 Jul 30. PubMed PMID: 23901080.
8. Huang PN, Shih SR. Update on enterovirus 71 infection. Curr Opin Virol. 2014 Apr;5:98-104. doi: 10.1016/j.coviro.2014.03.007. Epub 2014 Apr 12. Review. PubMed PMID: 24727707.
9. Liu YC, Kuo RL, Lin JY, Huang PN, Huang Y, Liu H, Arnold JJ, Chen SJ, Wang RY, Cameron CE, Shih SR. Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with Prp8. PLoS Pathog. 2014 Jun;10(6):e1004199. doi: 10.1371/journal.ppat.1004199. eCollection 2014 Jun. PubMed PMID: 24968230; PubMed Central PMCID: PMC4072778.
10. Weng KF, Hung CT, Hsieh PT, Li ML, Chen GW, Kung YA, Huang PN, Kuo RL, Chen LL, Lin JY, Wang RY, Chen SJ, Tang P, Horng JT, Huang HI, Wang JR, Ojcius DM, Brewer G, Shih SR. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res. 2014 Nov 10;42(20):12789-805. doi: 10.1093/nar/gku952. Epub 2014 Oct 28. PubMed PMID: 25352551; PubMed Central PMCID: PMC4227785.
11. Huang PN, Jheng JR, Arnold JJ, Wang JR, Cameron CE, Shih SR. UGGT1 enhances enterovirus 71 pathogenicity by promoting viral RNA synthesis and viral replication. PLoS Pathog. 2017 May;13(5):e1006375. doi: 10.1371/journal.ppat.1006375. eCollection 2017 May. PubMed PMID: 28545059; PubMed Central PMCID: PMC5435352.
12. Lee KM, Wu CC, Wu SE, Lin YH, Wang LT, Chang CR, Huang PN, Shih SR, Kuo RL. The RNA-dependent RNA polymerase of enterovirus A71 associates with ribosomal proteins and positively regulates protein translation. RNA Biol. 2020 Apr;17(4):608-622. doi: 10.1080/15476286.2020.1722448. Epub 2020 Feb 6. PubMed PMID: 32009553; PubMed Central PMCID: PMC7237135.
13. Huang PN, Wang HC, Hung HC, Tseng SN, Chang TY, Chou MY, Chen YJ, Wang YM, Shih SR, Hsu JT. Antiviral Activity of a Llama-Derived Single-Domain Antibody against Enterovirus A71. Antimicrob Agents Chemother. 2020 Apr 21;64(5). doi: 10.1128/AAC.01922-19. Print 2020 Apr 21. PubMed PMID: 32152074; PubMed Central PMCID: PMC7179613.
14. Huang CG, Lee KM, Hsiao MJ, Yang SL, Huang PN, Gong YN, Hsieh TH, Huang PW, Lin YJ, Liu YC, Tsao KC, Shih SR. Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19. J Clin Microbiol. 2020 Jul 23;58(8). doi: 10.1128/JCM.01068-20. Print 2020 Jul 23. PubMed PMID: 32518072; PubMed Central PMCID: PMC7383522.
15. Hung HC, Ke YY, Huang SY, Huang PN, Kung YA, Chang TY, Yen KJ, Peng TT, Chang SE, Huang CT, Tsai YR, Wu SH, Lee SJ, Lin JH, Liu BS, Sung WC, Shih SR, Chen CT, Hsu JT. Discovery of M Protease Inhibitors Encoded by SARS-CoV-2. Antimicrob Agents Chemother. 2020 Aug 20;64(9). doi: 10.1128/AAC.00872-20. Print 2020 Aug 20. PubMed PMID: 32669265; PubMed Central PMCID: PMC7449189.
16. Huang KA, Huang PN, Huang YC, Yang SL, Tsao KC, Chiu CH, Shih SR, Lin TY. Emergence of genotype C1 Enterovirus A71 and its link with antigenic variation of virus in Taiwan. PLoS Pathog. 2020 Sep;16(9):e1008857. doi: 10.1371/journal.ppat.1008857. eCollection 2020 Sep. PubMed PMID: 32936838; PubMed Central PMCID: PMC7521691.
17. Huang KA, Zhou D, Fry EE, Kotecha A, Huang PN, Yang SL, Tsao KC, Huang YC, Lin TY, Ren J, Stuart DI. Structural and functional analysis of protective antibodies targeting the threefold plateau of enterovirus 71. Nat Commun. 2020 Oct 16;11(1):5253. doi: 10.1038/s41467-020-19013-3. PubMed PMID: 33067459; PubMed Central PMCID: PMC7567869.
18. Gong YN, Tsao KC, Hsiao MJ, Huang CG, Huang PN, Huang PW, Lee KM, Liu YC, Yang SL, Kuo RL, Chen KF, Liu YC, Huang SY, Huang HI, Liu MT, Yang JR, Chiu CH, Yang CT, Chen GW, Shih SR. SARS-CoV-2 genomic surveillance in Taiwan revealed novel ORF8-deletion mutant and clade possibly associated with infections in Middle East. Emerg Microbes Infect. 2020 Dec;9(1):1457-1466. doi: 10.1080/22221751.2020.1782271. PubMed PMID: 32543353; PubMed Central PMCID: PMC7473175.
19. Wang HC, Hung HC, Huang PN, Kung YA, Tseng SN, Wang YM, Shih SR, Tsu-An Hsu J. Engineering a novel IgG-like bispecific antibody against enterovirus A71. Biochem Biophys Rep. 2020 Dec;24:100860. doi: 10.1016/j.bbrep.2020.100860. eCollection 2020 Dec. PubMed PMID: 34095549; PubMed Central PMCID: PMC8164134.
20. Wang HC, Huang PN, Hung HC, Tseng SN, Chang CC, Tsai YR, Wang YM, Shih SR, Hsu JT. Effect of a Neuropilin-1-Derived Virus Receptor Trap on Enterovirus A71 Infection In Vitro. Antimicrob Agents Chemother. 2020 Dec 16;65(1). doi: 10.1128/AAC.00695-20. Print 2020 Dec 16. PubMed PMID: 33139286; PubMed Central PMCID: PMC7927848.
21. Huang SY, Kung YA, Huang PN, Chang SY, Gong YN, Han YJ, Chiang HJ, Liu KT, Lee KM, Chang CY, Chang CC, Huang CG, Shih SR. Stability of SARS-CoV-2 Spike G614 Variant Surpasses That of the D614 Variant after Cold Storage. mSphere. 2021 Mar 31;6(2). doi: 10.1128/mSphere.00104-21. PubMed PMID: 33789940; PubMed Central PMCID: PMC8546686.
22. Mwale PF, Lee CH, Huang PN, Tseng SN, Shih SR, Huang HY, Leu SJ, Huang YJ, Chiang LC, Mao YC, Wang WC, Yang YY. In Vitro Characterization of Neutralizing Hen Antibodies to Coxsackievirus A16. Int J Mol Sci. 2021 Apr 16;22(8). doi: 10.3390/ijms22084146. PubMed PMID: 33923724; PubMed Central PMCID: PMC8074035.
23. Kiew LV, Chang CY, Huang SY, Wang PW, Heh CH, Liu CT, Cheng CH, Lu YX, Chen YC, Huang YX, Chang SY, Tsai HY, Kung YA, Huang PN, Hsu MH, Leo BF, Foo YY, Su CH, Hsu KC, Huang PH, Ng CJ, Kamarulzaman A, Yuan CJ, Shieh DB, Shih SR, Chung LY, Chang CC. Development of flexible electrochemical impedance spectroscopy-based biosensing platform for rapid screening of SARS-CoV-2 inhibitors. Biosens Bioelectron. 2021 Jul 1;183:113213. doi: 10.1016/j.bios.2021.113213. Epub 2021 Apr 3. PubMed PMID: 33857754; PubMed Central PMCID: PMC8018905.
24. Lin JY, Weng KF, Chang CK, Gong YN, Huang GJ, Lee HL, Chen YC, Huang CC, Lu JY, Huang PN, Chiang HJ, Chen CM, Shih SR. Enterovirus A71 Induces Neurological Diseases and Dynamic Variants in Oral Infection of Human SCARB2-Transgenic Weaned Mice. J Virol. 2021 Oct 13;95(21):e0089721. doi: 10.1128/JVI.00897-21. Epub 2021 Aug 11. PubMed PMID: 34379497; PubMed Central PMCID: PMC8513470.
25. Janissen R, Woodman A, Shengjuler D, Vallet T, Lee KM, Kuijpers L, Moustafa IM, Fitzgerald F, Huang PN, Perkins AL, Harki DA, Arnold JJ, Solano B, Shih SR, Vignuzzi M, Cameron CE, Dekker NH. Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses. Mol Cell. 2021 Nov 4;81(21):4467-4480.e7. doi: 10.1016/j.molcel.2021.10.003. Epub 2021 Oct 22. PubMed PMID: 34687604; PubMed Central PMCID: PMC8628313.
26. Kung YA, Chiang HJ, Li ML, Gong YN, Chiu HP, Hung CT, Huang PN, Huang SY, Wang PY, Hsu TA, Brewer G, Shih SR. Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis. mBio. 2022 Feb 22;13(1):e0271721. doi: 10.1128/mbio.02717-21. Epub 2022 Jan 18. PubMed PMID: 35038927; PubMed Central PMCID: PMC8764547.
27. Liu KT, Gong YN, Huang CG, Huang PN, Yu KY, Lee HC, Lee SC, Chiang HJ, Kung YA, Lin YT, Hsiao MJ, Huang PW, Huang SY, Wu HT, Wu CC, Kuo RL, Chen KF, Hung CT, Oguntuyo KY, Stevens CS, Kowdle S, Chiu HP, Lee B, Chen GW, Shih SR. Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model. mSphere. 2022 Feb 23;7(1):e0088321. doi: 10.1128/msphere.00883-21. Epub 2022 Feb 2. PubMed PMID: 35107336; PubMed Central PMCID: PMC8809379.
28. Liu KT, Han YJ, Wu GH, Huang KA, Huang PN. Overview of Neutralization Assays and International Standard for Detecting SARS-CoV-2 Neutralizing Antibody. Viruses. 2022 Jul 18;14(7). doi: 10.3390/v14071560. Review. PubMed PMID: 35891540; PubMed Central PMCID: PMC9322699.
29. Lee CH, Huang PN, Mwale PF, Wang WC, Leu SJ, Tseng SN, Shih SR, Chiang LC, Mao YC, Tsai BY, Dlamini NB, Nguyen TC, Tsai CH, Yang YY. The Bottlenecks of Preparing Virus Particles by Size Exclusion for Antibody Generation. Int J Mol Sci. 2022 Oct 26;23(21). doi: 10.3390/ijms232112967. PubMed PMID: 36361757; PubMed Central PMCID: PMC9653933.
30. Kung YA, Huang SY, Huang CG, Liu KT, Huang PN, Yu KY, Yang SL, Chen CP, Cheng CY, Lee IK, Lin SM, Chang HP, Lin YT, Liu YC, Chen GW, Shih SR. Factors influencing neutralizing antibody titers elicited by coronavirus disease 2019 vaccines. Microbes Infect. 2023 Jan-Feb;25(1-2):105044. doi: 10.1016/j.micinf.2022.105044. Epub 2022 Sep 9. PubMed PMID: 36096357; PubMed Central PMCID: PMC9461341.
31. Huang PN, Hsia SH, Huang KA, Chen CJ, Wang ET, Shih SR, Lin TY. Reflecting on the 1998 enterovirus outbreak: A 25-year retrospective and learned lessons. Biomed J. 2024 Mar 14;:100715. doi: 10.1016/j.bj.2024.100715. [Epub ahead of print] Review. PubMed PMID: 38492637.